Epigenomics: Sequencing the Methylome View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2013

AUTHORS

Martin Hirst

ABSTRACT

DNA methylation patterns are increasingly surveyed through methods that utilize massively parallel sequencing. Sequence-based assays developed to detect DNA methylation can be broadly divided into those that depend on affinity enrichment, chemical conversion, or enzymatic restriction. The DNA fragments resulting from these methods are uniformly subjected to library construction and massively parallel sequencing. The sequence reads are subsequently aligned to a reference genome and subjected to specialized analytical tools to extract the underlying methylation signature. This chapter will outline these emerging techniques. More... »

PAGES

39-54

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-62703-281-0_3

DOI

http://dx.doi.org/10.1007/978-1-62703-281-0_3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012892923

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23412782


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