Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2013

AUTHORS

Nirupama K. Shevde , Amber A. Mael

ABSTRACT

Embryoid bodies (EBs) can be generated by culturing human pluripotent stem cells in ultra-low attachment culture vessels, under conditions that are adverse to pluripotency and proliferation. EBs generated in suspension cultures are capable of differentiating into cells of the ectoderm, mesoderm, and endoderm. In this chapter, we describe techniques for generation of EBs from human pluripotent stem cells. Once formed, the EBs can then be dissociated using specific enzymes to acquire a single cell population that has the potential to differentiate into cells of all three germ layers. This population can then be cultured in specialized conditions to obtain progenitor cells of specific lineages. Pure populations of progenitor cells generated on a large scale basis can be used for research, drug discovery/development, and cellular transplantation therapy. More... »

PAGES

535-546

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-62703-128-8_33

DOI

http://dx.doi.org/10.1007/978-1-62703-128-8_33

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049460135

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23179854


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