Transcriptome Analysis Using RNA-Seq View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Wieteke A. M. Hoeijmakers , Richárd Bártfai , Hendrik G. Stunnenberg

ABSTRACT

Transcriptome analysis by next-generation sequencing (RNA-seq) allows investigation of a transcriptome at unsurpassed resolution. One major benefit is that RNA-seq is independent of a priori knowledge on the sequence under investigation, thereby also allowing analysis of poorly characterized Plasmodium species. Here we provide a detailed protocol for RNA isolation and fragmentation, ribosomal RNA depletion, and cDNA synthesis that enables the preparation of a sequencing library from 1 to 2 μg of total RNA. Although we focus our discussion on the quantitative measurement of gene expression, this protocol is suited for many applications of RNA-seq and allows analysis of most RNA species. More... »

PAGES

221-39

References to SciGraph publications

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  • 2010-12. From RNA-seq reads to differential expression results in GENOME BIOLOGY
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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-62703-026-7_15

    DOI

    http://dx.doi.org/10.1007/978-1-62703-026-7_15

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1002241423

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22990781


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