Omics in Stem Cell Therapy: The Road Ahead View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Kyunghee Byun , Goo-Bo Jeong , Trevor N. Collingwood , Bonghee Lee

ABSTRACT

Understanding self-renewal and differentiation of stem cells at molecular levels is essential for both basic research and clinical applications of stem cells. The complexity of stem cell fate is controlled by the sophisticated mechanisms of temporal and spatial gene expression and protein function. Thus, the capacity to control a gene’s expression, build cellular reporters that reflect the activity state of lineage-specific endogenous genes, and either create or repair disease alleles is essential for realizing the potential of stem cell technology. In this chapter, we introduce zinc finger nucleases (ZFNs) as a tool to efficiently and selectively manipulate a given gene at the endogenous locus for advancing both our understanding as well as therapeutic application of stem cell biology. Proteins too are key players in the cell and often serve as biomarkers. They have diverse features that are not predictable from gene sequences or from the level of transcripts. Major breakthroughs in stem cell research were made by the identification of protein biomarkers such as colony-stimulating factors (CSFs) and cell-surface CD molecules. Recently, the Human Proteome Organization (HUPO) and Asia Oceanic HUPO (AOHUPO) launched initiatives for systematic MS-based proteomics as well as a gene coded proteomic approach to understanding stem cell differentiation. These studies will shed new light on stem cell biology and accelerate clinical applications of stem cells. More... »

PAGES

227-243

Book

TITLE

Advances in Stem Cell Research

ISBN

978-1-61779-939-6
978-1-61779-940-2

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-940-2_13

DOI

http://dx.doi.org/10.1007/978-1-61779-940-2_13

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035362507


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