Ontology type: schema:Chapter
2012-04-24
AUTHORSNils Arrigo , Rolf Holderegger , Nadir Alvarez
ABSTRACTAmplified Fragment Length Polymorphisms (AFLPs) are a cheap and efficient protocol for generating large sets of genetic markers. This technique has become increasingly used during the last decade in various fields of biology, including population genomics, phylogeography, and genome mapping. Here, we present RawGeno, an R library dedicated to the automated scoring of AFLPs (i.e., the coding of electropherogram signals into ready-to-use datasets). Our program includes a complete suite of tools for binning, editing, visualizing, and exporting results obtained from AFLP experiments. RawGeno can either be used with command lines and program analysis routines or through a user-friendly graphical user interface. We describe the whole RawGeno pipeline along with recommendations for (a) setting the analysis of electropherograms in combination with PeakScanner, a program freely distributed by Applied Biosystems; (b) performing quality checks; (c) defining bins and proceeding to scoring; (d) filtering nonoptimal bins; and (e) exporting results in different formats. More... »
PAGES155-175
Data Production and Analysis in Population Genomics
ISBN
978-1-61779-869-6
978-1-61779-870-2
http://scigraph.springernature.com/pub.10.1007/978-1-61779-870-2_10
DOIhttp://dx.doi.org/10.1007/978-1-61779-870-2_10
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1006625244
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/22665281
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