Microarray-Based Gene Profiling Analysis of Müller Glia-Derived Retinal Stem Cells in Light-Damaged Retinas from Adult Zebrafish View Full Text


Ontology type: schema:Chapter      Open Access: True


Chapter Info

DATE

2012-05-10

AUTHORS

Zhao Qin , Pamela A. Raymond

ABSTRACT

Microarray-based gene profiling has become an important technique to measure changes in gene expression on a genome-wide scale. Recently, cell-specific microarrays have been reported to study changes in gene expression of a particular cell type in several model organisms. Here, we describe a protocol to prepare RNA samples for microarray analysis of isolated Müller glia-derived retinal stem cells from light-damaged adult zebrafish expressing a fluorescent marker in Müller cells using enzymatic retinal dissociation followed by fluorescence-activated cell sorting (FACS). More... »

PAGES

255-261

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-848-1_18

DOI

http://dx.doi.org/10.1007/978-1-61779-848-1_18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002132994

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22688712


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