Microarray-Based Gene Profiling Analysis of Müller Glia-Derived Retinal Stem Cells in Light-Damaged Retinas from Adult Zebrafish View Full Text


Ontology type: schema:Chapter      Open Access: True


Chapter Info

DATE

2012-05-10

AUTHORS

Zhao Qin , Pamela A. Raymond

ABSTRACT

Microarray-based gene profiling has become an important technique to measure changes in gene expression on a genome-wide scale. Recently, cell-specific microarrays have been reported to study changes in gene expression of a particular cell type in several model organisms. Here, we describe a protocol to prepare RNA samples for microarray analysis of isolated Müller glia-derived retinal stem cells from light-damaged adult zebrafish expressing a fluorescent marker in Müller cells using enzymatic retinal dissociation followed by fluorescence-activated cell sorting (FACS). More... »

PAGES

255-261

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-848-1_18

DOI

http://dx.doi.org/10.1007/978-1-61779-848-1_18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002132994

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22688712


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flow Cytometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Profiling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Light", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neuroglia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oligonucleotide Array Sequence Analysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Retina", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Zebrafish", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY, USA", 
          "id": "http://www.grid.ac/institutes/grid.137628.9", 
          "name": [
            "Department of Molecular, Cellular, and Developmental Biology, University of Michigan College of Literature, Science, and the Arts, Ann Arbor, MI, USA", 
            "Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Qin", 
        "givenName": "Zhao", 
        "id": "sg:person.0621721035.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0621721035.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular, Cellular, and Developmental Biology, University of Michigan College of Literature, Science, and the Arts, Ann Arbor, MI, USA", 
          "id": "http://www.grid.ac/institutes/grid.214458.e", 
          "name": [
            "Department of Molecular, Cellular, and Developmental Biology, University of Michigan College of Literature, Science, and the Arts, Ann Arbor, MI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Raymond", 
        "givenName": "Pamela A.", 
        "id": "sg:person.0772236112.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772236112.67"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2012-05-10", 
    "datePublishedReg": "2012-05-10", 
    "description": "Microarray-based gene profiling has become an important technique to measure changes in gene expression on a genome-wide scale. Recently, cell-specific microarrays have been reported to study changes in gene expression of a particular cell type in several model organisms. Here, we describe a protocol to prepare RNA samples for microarray analysis of isolated M\u00fcller glia-derived retinal stem cells from light-damaged adult zebrafish expressing a fluorescent marker in M\u00fcller cells using enzymatic retinal dissociation followed by fluorescence-activated cell sorting (FACS).", 
    "editor": [
      {
        "familyName": "Wang", 
        "givenName": "Shu-Zhen", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-61779-848-1_18", 
    "isAccessibleForFree": true, 
    "isPartOf": {
      "isbn": [
        "978-1-61779-847-4", 
        "978-1-61779-848-1"
      ], 
      "name": "Retinal Development", 
      "type": "Book"
    }, 
    "keywords": [
      "fluorescence-activated cell sorting", 
      "retinal stem cells", 
      "adult zebrafish", 
      "gene expression", 
      "genome-wide scale", 
      "gene profiling analyses", 
      "stem cells", 
      "M\u00fcller glia", 
      "particular cell type", 
      "model organisms", 
      "microarray analysis", 
      "gene profiling", 
      "profiling analysis", 
      "cell types", 
      "RNA samples", 
      "cell sorting", 
      "zebrafish", 
      "microarray", 
      "fluorescent markers", 
      "cells", 
      "expression", 
      "M\u00fcller cells", 
      "glia", 
      "organisms", 
      "profiling", 
      "sorting", 
      "markers", 
      "changes", 
      "analysis", 
      "dissociation", 
      "retina", 
      "light", 
      "types", 
      "important technique", 
      "protocol", 
      "samples", 
      "scale", 
      "technique"
    ], 
    "name": "Microarray-Based Gene Profiling Analysis of M\u00fcller Glia-Derived Retinal Stem Cells in Light-Damaged Retinas from Adult Zebrafish", 
    "pagination": "255-261", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1002132994"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-61779-848-1_18"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22688712"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-61779-848-1_18", 
      "https://app.dimensions.ai/details/publication/pub.1002132994"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2022-12-01T06:47", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/chapter/chapter_149.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1007/978-1-61779-848-1_18"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-61779-848-1_18'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-61779-848-1_18'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-61779-848-1_18'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-61779-848-1_18'


 

This table displays all metadata directly associated to this object as RDF triples.

148 TRIPLES      22 PREDICATES      72 URIs      65 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-61779-848-1_18 schema:about N08047f2eff8e432d9e31912cb5ad1c3c
2 N2580c8857a7f4156bf11d2ac63c38fd0
3 N25cdebebe00d453a9bcd52dbcdfb1dfb
4 N2cb7203f6bef4f06a60d65b7d4a084df
5 N472e6045117e482daf100adea8b6ba58
6 N501bf033c2704248964ed8f6f4271189
7 N8db1ae472d8f45a3860c834a0f7141e2
8 Nc8012f5bfc90490b8d1d5d810befe626
9 Needd3018f6f842cc8e6b3c81d0595cca
10 anzsrc-for:06
11 anzsrc-for:0604
12 schema:author Nd083abd02bc44b3fb22dbaaf48053098
13 schema:datePublished 2012-05-10
14 schema:datePublishedReg 2012-05-10
15 schema:description Microarray-based gene profiling has become an important technique to measure changes in gene expression on a genome-wide scale. Recently, cell-specific microarrays have been reported to study changes in gene expression of a particular cell type in several model organisms. Here, we describe a protocol to prepare RNA samples for microarray analysis of isolated Müller glia-derived retinal stem cells from light-damaged adult zebrafish expressing a fluorescent marker in Müller cells using enzymatic retinal dissociation followed by fluorescence-activated cell sorting (FACS).
16 schema:editor Nead676bcb5f046e2adf48340670e4f51
17 schema:genre chapter
18 schema:isAccessibleForFree true
19 schema:isPartOf N7cee50d53c2a43118803481691d9fcc8
20 schema:keywords Müller cells
21 Müller glia
22 RNA samples
23 adult zebrafish
24 analysis
25 cell sorting
26 cell types
27 cells
28 changes
29 dissociation
30 expression
31 fluorescence-activated cell sorting
32 fluorescent markers
33 gene expression
34 gene profiling
35 gene profiling analyses
36 genome-wide scale
37 glia
38 important technique
39 light
40 markers
41 microarray
42 microarray analysis
43 model organisms
44 organisms
45 particular cell type
46 profiling
47 profiling analysis
48 protocol
49 retina
50 retinal stem cells
51 samples
52 scale
53 sorting
54 stem cells
55 technique
56 types
57 zebrafish
58 schema:name Microarray-Based Gene Profiling Analysis of Müller Glia-Derived Retinal Stem Cells in Light-Damaged Retinas from Adult Zebrafish
59 schema:pagination 255-261
60 schema:productId N0eb97ff414cf43bc9a49337f8165c1f8
61 N3a62082082db405b910a04f94ff33110
62 N8304b4ec62194cb99634059b95e15516
63 schema:publisher Nb8096969e8f441359203e9b3afef2487
64 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002132994
65 https://doi.org/10.1007/978-1-61779-848-1_18
66 schema:sdDatePublished 2022-12-01T06:47
67 schema:sdLicense https://scigraph.springernature.com/explorer/license/
68 schema:sdPublisher Ne0fc9f6e91d140d2a010623a23533355
69 schema:url https://doi.org/10.1007/978-1-61779-848-1_18
70 sgo:license sg:explorer/license/
71 sgo:sdDataset chapters
72 rdf:type schema:Chapter
73 N08047f2eff8e432d9e31912cb5ad1c3c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Retina
75 rdf:type schema:DefinedTerm
76 N0eb97ff414cf43bc9a49337f8165c1f8 schema:name doi
77 schema:value 10.1007/978-1-61779-848-1_18
78 rdf:type schema:PropertyValue
79 N2580c8857a7f4156bf11d2ac63c38fd0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Animals
81 rdf:type schema:DefinedTerm
82 N25cdebebe00d453a9bcd52dbcdfb1dfb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Light
84 rdf:type schema:DefinedTerm
85 N2cb7203f6bef4f06a60d65b7d4a084df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Flow Cytometry
87 rdf:type schema:DefinedTerm
88 N3a62082082db405b910a04f94ff33110 schema:name dimensions_id
89 schema:value pub.1002132994
90 rdf:type schema:PropertyValue
91 N472e6045117e482daf100adea8b6ba58 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Stem Cells
93 rdf:type schema:DefinedTerm
94 N501bf033c2704248964ed8f6f4271189 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Oligonucleotide Array Sequence Analysis
96 rdf:type schema:DefinedTerm
97 N7cee50d53c2a43118803481691d9fcc8 schema:isbn 978-1-61779-847-4
98 978-1-61779-848-1
99 schema:name Retinal Development
100 rdf:type schema:Book
101 N8304b4ec62194cb99634059b95e15516 schema:name pubmed_id
102 schema:value 22688712
103 rdf:type schema:PropertyValue
104 N8db1ae472d8f45a3860c834a0f7141e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Gene Expression Profiling
106 rdf:type schema:DefinedTerm
107 N980f923b41e441e7b51e08b47aa95b71 schema:familyName Wang
108 schema:givenName Shu-Zhen
109 rdf:type schema:Person
110 Nb8096969e8f441359203e9b3afef2487 schema:name Springer Nature
111 rdf:type schema:Organisation
112 Nc8012f5bfc90490b8d1d5d810befe626 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Zebrafish
114 rdf:type schema:DefinedTerm
115 Nd083abd02bc44b3fb22dbaaf48053098 rdf:first sg:person.0621721035.27
116 rdf:rest Nfe4f085c3624439d9f6c44ec26136570
117 Ne0fc9f6e91d140d2a010623a23533355 schema:name Springer Nature - SN SciGraph project
118 rdf:type schema:Organization
119 Nead676bcb5f046e2adf48340670e4f51 rdf:first N980f923b41e441e7b51e08b47aa95b71
120 rdf:rest rdf:nil
121 Needd3018f6f842cc8e6b3c81d0595cca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Neuroglia
123 rdf:type schema:DefinedTerm
124 Nfe4f085c3624439d9f6c44ec26136570 rdf:first sg:person.0772236112.67
125 rdf:rest rdf:nil
126 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
127 schema:name Biological Sciences
128 rdf:type schema:DefinedTerm
129 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
130 schema:name Genetics
131 rdf:type schema:DefinedTerm
132 sg:person.0621721035.27 schema:affiliation grid-institutes:grid.137628.9
133 schema:familyName Qin
134 schema:givenName Zhao
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0621721035.27
136 rdf:type schema:Person
137 sg:person.0772236112.67 schema:affiliation grid-institutes:grid.214458.e
138 schema:familyName Raymond
139 schema:givenName Pamela A.
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772236112.67
141 rdf:type schema:Person
142 grid-institutes:grid.137628.9 schema:alternateName Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY, USA
143 schema:name Department of Molecular, Cellular, and Developmental Biology, University of Michigan College of Literature, Science, and the Arts, Ann Arbor, MI, USA
144 Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY, USA
145 rdf:type schema:Organization
146 grid-institutes:grid.214458.e schema:alternateName Department of Molecular, Cellular, and Developmental Biology, University of Michigan College of Literature, Science, and the Arts, Ann Arbor, MI, USA
147 schema:name Department of Molecular, Cellular, and Developmental Biology, University of Michigan College of Literature, Science, and the Arts, Ann Arbor, MI, USA
148 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...