In-Gel Staining of Proteins in Native Polyacrylamide Gel Electrophoresis Using Meso-Tetrakis(4-Sulfonatophenyl) Porphyrin View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

K. Divakar , G. Nandhini Devi , Pennathur Gautam

ABSTRACT

Protein identification in polyacrylamide gel electrophoresis (PAGE) requires post-electrophoretic steps like fixing, staining, and destaining of the gel, which are time-consuming and cumbersome. A new method for direct visualization of protein bands in PAGE has been developed using meso-tetrakis(4-sulfonatophenyl)porphyrin (TPPS) as a dye without the need for any post-electrophoretic steps; thus, separation and recovery of enzymes become much easier for further analysis. Activity staining was carried out to show that the biochemical activity of the enzymes was preserved after electrophoresis. More... »

PAGES

579-84

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-821-4_52

DOI

http://dx.doi.org/10.1007/978-1-61779-821-4_52

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046270788

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22585523


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