Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Leyre Brizuela , Olivier Cuvillier

ABSTRACT

Sphingolipids (ceramide, sphingosine, and sphingosine-1-phosphate) are bioactive lipids with important biological functions in proliferation, apoptosis, angiogenesis, and inflammation. Herein, we describe easy and rapid biochemical methods with the use of radiolabeled molecules ((3)H, (32)P) for their mass determination. Quantitation of sphingosine kinase-1 activity, the most studied isoform, is also included.

PAGES

1-20

References to SciGraph publications

  • 1998. Sphingosine Kinase in PHOSPHOLIPID SIGNALING PROTOCOLS
  • 2003-05. Sphingosine-1-phosphate: an enigmatic signalling lipid in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2008-02. Principles of bioactive lipid signalling: lessons from sphingolipids in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 1996-06. Suppression of ceramide-mediated programmed cell death by sphingosine-1-phosphate in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-61779-800-9_1

    DOI

    http://dx.doi.org/10.1007/978-1-61779-800-9_1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1051600536

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22528435


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