Agrobacterium-Mediated Transformation of Brachypodium distachyon View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012-01-09

AUTHORS

Vera Thole , Philippe Vain

ABSTRACT

Brachypodium distachyon is an attractive genomics and biological model system for grass research. Recently, the complete annotated genome sequence of the diploid line Bd21 has been released. Genetic transformation technologies are critical for the discovery and validation of gene function in Brachypodium. Here, we describe an efficient procedure enabling the Agrobacterium-mediated transformation of a range of diploid and polyploid genotypes of Brachypodium. The procedure relies on the transformation of compact embryogenic calli derived from immature embryos using either chemical selection alone or a combination of chemical and visual screening of transformed tissues and plants. Transformation efficiencies of around 20% can routinely be achieved using this protocol. In the context of the BrachyTAG programme (BrachyTAG.org), this procedure made possible the mass production of Bd21T-DNA mutant plant lines. More... »

PAGES

137-149

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-558-9_12

DOI

http://dx.doi.org/10.1007/978-1-61779-558-9_12

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019492486

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22351005


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