DNA Extraction from Fossil Eggshell View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Charlotte L. Oskam , Michael Bunce

ABSTRACT

Avian eggshell fragments recovered from both paleontological and archaeological deposits contain a cache of well-preserved ancient DNA. Here, we describe an extraction protocol that has been optimized to maximize the recovery of ancient DNA from fossil eggshell and minimize the co-purification of PCR inhibitors. In this method, fossil eggshell fragments are powdered, then digested and heated to release DNA from the calcite matrix. The digest then undergoes a concentration step before purification and washing using silica columns. The method has been used to recover aDNA from the eggshell of many avian species including moa, elephant birds, and emu, up to 19,000 years old. More... »

PAGES

65-70

Book

TITLE

Ancient DNA

ISBN

978-1-61779-515-2
978-1-61779-516-9

Author Affiliations

From Grant

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-516-9_9

DOI

http://dx.doi.org/10.1007/978-1-61779-516-9_9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028075217

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22237523


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