Case Study: Ancient Sloth DNA Recovered from Hairs Preserved in Paleofeces View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Andrew A. Clack , Ross D. E. MacPhee , Hendrik N. Poinar

ABSTRACT

Ancient hair, which has proved to be an excellent source of well-preserved ancient DNA, is often preserved in paleofeces. Here, we separate and wash hair shafts preserved in a paleofecal specimen believed to be from a Darwin's ground sloth, Mylodon darwinii. After extracting DNA from the recovered and cleaned hair using a protocol optimized for DNA extraction from keratinous substrates, we amplify 12S and 16S rDNA sequences from the DNA extract. As expected, the recovered sequences most closely match previously published sequences of M. darwinii. Our results demonstrate that hair preserved in paleofeces, even from temperate cave environments, is an effective source of ancient DNA. More... »

PAGES

51-6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-516-9_7

DOI

http://dx.doi.org/10.1007/978-1-61779-516-9_7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021592611

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22237521


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