Extraction of DNA from Paleofeces View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Melanie Kuch , Hendrik Poinar

ABSTRACT

Paleofeces are the nonmineralized remains of dung from extant and extinct fauna. They represent a surprisingly large proportion of fossil remains recovered from cave sites across the world. Paleofeces contain the DNA of the defecator as well as the DNA of ingested plant and animal remains. To successfully extract DNA from paleofeces, a balance must be achieved between the minimization of DNA loss during extraction and the removal of coeluates that would otherwise inhibit the Taq DNA polymerase during downstream applications. Here we present a simplified version of a protocol to extract DNA from paleofecal remains. More... »

PAGES

37-42

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-516-9_5

DOI

http://dx.doi.org/10.1007/978-1-61779-516-9_5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003990320

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22237519


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