Case Study: Recovery of Ancient Nuclear DNA from Toe Pads of the Extinct Passenger Pigeon View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Tara L. Fulton , Stephen M. Wagner , Beth Shapiro

ABSTRACT

A variety of DNA extraction methods have been employed successfully to extract DNA from museum specimens. Toe pads are a common source of ancient DNA in birds, as they are generally not an informative character and can be removed without significant destruction of precious specimens. However, the DNA in these tissues is often highly degraded, both by natural postmortem decay and due to treatment by preservatives. In this case study chapter, we describe the use of both a commercial DNA extraction method and a silica-based method to extract ancient DNA from desiccated toe pads from the extinct passenger pigeon, Ectopistes migratorius. Successful amplification of nuclear DNA was achieved from both methods, representing the first nuclear DNA sequence recovered from this extinct species. We describe simple modifications to both protocols that we employed during the DNA extraction process. More... »

PAGES

29-35

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-516-9_4

DOI

http://dx.doi.org/10.1007/978-1-61779-516-9_4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026216524

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22237518


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