A Phenol–Chloroform Protocol for Extracting DNA from Ancient Samples View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Ross Barnett , Greger Larson

ABSTRACT

The myriad downstream applications of ancient DNA (aDNA) analysis all ultimately require that sequence data are generated from extracts of ancient material. DNA extraction from tissues known to contain preserved biomolecules (e.g. teeth, hair, tissue, bone) relies on subtle modifications of a basic technique that has been in use for nearly two decades. Multiple DNA extraction protocols have been introduced, with varying levels of success depending on tissue type and the long-term preservation environment to which the ancient tissue was exposed. Here, we describe the phenol-chloroform method for extracting aDNA from any tissue type. This commonly employed method allows for the recovery of total nucleic acid content with minimal loss of low molecular weight double-stranded DNA. More... »

PAGES

13-19

References to SciGraph publications

  • 2007-07. Ancient DNA extraction from bones and teeth in NATURE PROTOCOLS
  • 1993-04. Instability and decay of the primary structure of DNA in NATURE
  • 1989-11. Ancient bone DNA amplified in NATURE
  • 1994-06. Ancient DNA: Methodological challenges in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 2001-05. Ancient DNA in NATURE REVIEWS GENETICS
  • 1996-07. An agent cleaving glucose-derived protein crosslinks in vitro and in vivo in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-61779-516-9_2

    DOI

    http://dx.doi.org/10.1007/978-1-61779-516-9_2

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1040904652

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22237516


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