Generating Barcoded Libraries for Multiplex High-Throughput Sequencing View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Michael Knapp , Mathias Stiller , Matthias Meyer

ABSTRACT

Molecular barcoding is an essential tool to use the high throughput of next generation sequencing platforms optimally in studies involving more than one sample. Various barcoding strategies allow for the incorporation of short recognition sequences (barcodes) into sequencing libraries, either by ligation or polymerase chain reaction (PCR). Here, we present two approaches optimized for generating barcoded sequencing libraries from low copy number extracts and amplification products typical of ancient DNA studies. More... »

PAGES

155-70

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-516-9_19

DOI

http://dx.doi.org/10.1007/978-1-61779-516-9_19

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000836912

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22237533


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