Quantitative Real-Time PCR in aDNA Research View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Michael Bunce , Charlotte L. Oskam , Morten E. Allentoft

ABSTRACT

Quantitative real-time PCR (qPCR) is a technique that is widely used in the field of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing. In this chapter, we outline factors that need to be considered when developing efficient SYBR Green qPCR assays. We describe how to setup qPCR standards of known copy number and provide some useful tips regarding interpretation of qPCR data generated from aDNA templates. More... »

PAGES

121-32

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-516-9_16

DOI

http://dx.doi.org/10.1007/978-1-61779-516-9_16

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1033314689

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22237530


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