Case Study: Ancient DNA Recovered from Pleistocene-Age Remains of a Florida Armadillo View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Brandon Letts , Beth Shapiro

ABSTRACT

Warm, humid regions are not ideal for long-term DNA preservation. Consequently, little ancient DNA research has been carried out involving taxa that lived in, for example, tropical and subtropical regions. Those studies that have isolated ancient DNA from warm environments have mostly been limited to the most recent several thousand years. Here, we discuss an ancient DNA experiment in which we attempt to amplify mitochondrial DNA from remains of armadillo, glyptodont, and pampathere from sites in Florida, USA, all believed to be around 10,000-12,000 years old. We were successful in recovering DNA from only one of these samples. However, based on the amount and distribution of DNA damage, the ancient DNA recovered was well-preserved despite the age and preservation environment. In this case study chapter, we discuss the experimental procedure we used to characterize the DNA from the Floridian samples, focusing on challenges of working with ancient specimens from warm environments and steps taken to confirm the authenticity of the recovered sequence. More... »

PAGES

87-92

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-516-9_12

DOI

http://dx.doi.org/10.1007/978-1-61779-516-9_12

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027373342

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22237526


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