Ancient DNA Extraction from Plants View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Logan Kistler

ABSTRACT

A variety of protocols for DNA extraction from archaeological and paleobotanical plant specimens have been proposed. This is not surprising given the range of taxa and tissue types that may be preserved and the variety of conditions in which that preservation may take place. Commercially available DNA extraction kits can be used to recover ancient plant DNA, but modifications to standard approaches are often necessary to improve yield. In this chapter, I describe two protocols for extracting DNA from small amounts of ancient plant tissue. The CTAB protocol, which I recommend for use with single seeds, utilizes an incubation period in extraction buffer and subsequent chloroform extraction followed by DNA purification and suspension. The PTB protocol, which I recommend for use with gourd rind and similar tissues, utilizes an overnight incubation of pulverized tissue in extraction buffer, removal of the tissue by centrifugation, and DNA extraction from the buffer using commercial plant DNA extraction kits. More... »

PAGES

71-79

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-516-9_10

DOI

http://dx.doi.org/10.1007/978-1-61779-516-9_10

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028602211

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22237524


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