MAPPIT as a High-Throughput Screening Assay for Modulators of Protein–Protein Interactions in HIV and HCV View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Bertrand Van Schoubroeck , Koen Van Acker , Géry Dams , Dirk Jochmans , Reginald Clayton , Jan Martin Berke , Sam Lievens , José Van der Heyden , Jan Tavernier

ABSTRACT

The discovery of novel antivirals for HIV and HCV has been a focus of intensive research for many years. Where the inhibition of critical viral enzymes by small molecules has proven effective for many viruses, there is considerable merit in pursuing protein-protein interactions (PPIs) as targets for therapeutic intervention. The mammalian protein-protein interaction trap (MAPPIT) is a two-hybrid system used for the study of PPIs. The bait and prey proteins are linked to deficient cytokine receptor chimeras, where the bait and prey interaction and subsequent ligand stimulation restores JAK-STAT signaling, resulting in reporter gene expression controlled by a STAT3-responsive promoter. We report the use of MAPPIT as a high-throughput screening assay for the discovery of inhibitors or stimulators of the Vif-APOBEC3G interaction and the reverse transcriptase heterodimerization (RTp66-RTp51) for HIV and the NS4A-NS3 interaction for HCV. More... »

PAGES

295-307

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-455-1_18

DOI

http://dx.doi.org/10.1007/978-1-61779-455-1_18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011218176

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22218868


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