Recombinant Protein Expression in Milk of Livestock Species View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Zsuzsanna Bösze , László Hiripi

ABSTRACT

Producing complex recombinant proteins in the milk of transgenic animals offers several advantages: large amounts of proteins can be obtained, and in most cases, these proteins are properly folded, assembled, cleaved, and glycosylated. The level of expression of foreign genes in the mammalian gland cannot be predicted in all cases, and appropriate vectors must be used. The main elements of these vectors are as follows: a well-characterized specific promoter, the coding region of the gene of interest, preferably with a homologous or heterologous intron, to improve transcription efficiency, and an insulator or boundary element to counteract the chromosomal position effects at the integration site. Once high expression levels are achieved, and the recombinant protein is purified, an essential step in the analysis of the final product is determining its degree of glycosylation. This is an important readout because it can affect among other parameters the stability and immunogenicity of the recombinant protein. More... »

PAGES

629-41

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-433-9_34

DOI

http://dx.doi.org/10.1007/978-1-61779-433-9_34

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026680557

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22160924


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals, Genetically Modified", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biotechnology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomes, Artificial, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomes, Artificial, Yeast", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Livestock", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microinjections", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Milk", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Species Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transgenes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Agricultural Biotechnology Institute", 
          "id": "https://www.grid.ac/institutes/grid.417744.5", 
          "name": [
            "Agricultural Biotechnology Center, G\u00f6d\u00f6ll, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "B\u00f6sze", 
        "givenName": "Zsuzsanna", 
        "id": "sg:person.0600053006.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600053006.69"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Agricultural Biotechnology Institute", 
          "id": "https://www.grid.ac/institutes/grid.417744.5", 
          "name": [
            "Agricultural Biotechnology Center, G\u00f6d\u00f6ll, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hiripi", 
        "givenName": "L\u00e1szl\u00f3", 
        "id": "sg:person.01320374062.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320374062.84"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/glycob/10.5.477", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001266444"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1098-2795(199909)54:1<17::aid-mrd3>3.0.co;2-n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003306980"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11248-006-9015-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003563056", 
          "https://doi.org/10.1007/s11248-006-9015-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1018442124584", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005524006", 
          "https://doi.org/10.1023/a:1018442124584"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/10425170601017145", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006597680"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.988502", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007648644"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/gene.10249", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008837647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0003-2697(02)00273-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009026616"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0003-2697(02)00273-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009026616"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jchromb.2009.04.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010130547"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/glycob/cwm015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011623040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1295-1452", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011850752", 
          "https://doi.org/10.1038/nbt1295-1452"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/glycob/cwh010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014083759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2105-8-71", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014726920", 
          "https://doi.org/10.1186/1471-2105-8-71"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2105-8-71", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014726920", 
          "https://doi.org/10.1186/1471-2105-8-71"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/mrd.90007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015847382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1067081", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018333078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/biot.200800069", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019425468"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cimid.2007.11.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021178827"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2002-04-1104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021476120"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11248-007-9137-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022392937", 
          "https://doi.org/10.1007/s11248-007-9137-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0397-239", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022614305", 
          "https://doi.org/10.1038/nbt0397-239"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1297-9686-34-1-117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022879721", 
          "https://doi.org/10.1186/1297-9686-34-1-117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.2000.3013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025375587"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/10826069308544565", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026487676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.25.6.2260-2272.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027050792"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/8.12.2145", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027096024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)80052-g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030579809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0003453", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031242823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/dvdy.22051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031623864"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/dvdy.22051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031623864"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0997-859", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031958037", 
          "https://doi.org/10.1038/nbt0997-859"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiotec.2006.10.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035592352"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.abb.2007.11.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036163300"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1098-2795(199904)52:4<414::aid-mrd10>3.0.co;2-s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038575760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.0030007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039517858"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0163-7258(03)00069-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040880042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0163-7258(03)00069-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040880042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(87)90584-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041471577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cimid.2007.11.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041897979"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.theriogenology.2006.09.034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042529548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2004.10.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043611646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2004.10.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043611646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0192-74", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043711637", 
          "https://doi.org/10.1038/nbt0192-74"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0070-2153(07)80009-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047834280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/27.6.1555", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048868488"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/glycob/cwn035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049370417"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.01326-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052357308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11248-009-9271-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052514860", 
          "https://doi.org/10.1007/s11248-009-9271-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11248-009-9271-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052514860", 
          "https://doi.org/10.1007/s11248-009-9271-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.275.2.759", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053656874"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0022029900004386", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054049010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9603783", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211762"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9603783", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211762"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/104454903321112488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059203798"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/15362300152725936", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059214841"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.4004805", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060407529"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1140871", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062455912"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1615/critreveukargeneexpr.v10.i2.60", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068130907"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3168/jds.2008-1189", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077815204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3168/jds.2008-1189", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077815204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083274025", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012", 
    "datePublishedReg": "2012-01-01", 
    "description": "Producing complex recombinant proteins in the milk of transgenic animals offers several advantages: large amounts of proteins can be obtained, and in most cases, these proteins are properly folded, assembled, cleaved, and glycosylated. The level of expression of foreign genes in the mammalian gland cannot be predicted in all cases, and appropriate vectors must be used. The main elements of these vectors are as follows: a well-characterized specific promoter, the coding region of the gene of interest, preferably with a homologous or heterologous intron, to improve transcription efficiency, and an insulator or boundary element to counteract the chromosomal position effects at the integration site. Once high expression levels are achieved, and the recombinant protein is purified, an essential step in the analysis of the final product is determining its degree of glycosylation. This is an important readout because it can affect among other parameters the stability and immunogenicity of the recombinant protein.", 
    "editor": [
      {
        "familyName": "Lorence", 
        "givenName": "Argelia", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-61779-433-9_34", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-61779-432-2", 
        "978-1-61779-433-9"
      ], 
      "name": "Recombinant Gene Expression", 
      "type": "Book"
    }, 
    "name": "Recombinant Protein Expression in Milk of Livestock Species", 
    "pagination": "629-41", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-61779-433-9_34"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7f1a6bb4bc36589c827978df8ce15862d743c72214e1380288eb986779cf8747"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1026680557"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22160924"
        ]
      }
    ], 
    "publisher": {
      "location": "Totowa, NJ", 
      "name": "Humana Press", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-61779-433-9_34", 
      "https://app.dimensions.ai/details/publication/pub.1026680557"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-16T00:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000259.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-61779-433-9_34"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-61779-433-9_34'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-61779-433-9_34'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-61779-433-9_34'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-61779-433-9_34'


 

This table displays all metadata directly associated to this object as RDF triples.

307 TRIPLES      23 PREDICATES      97 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-61779-433-9_34 schema:about N07182ad3a1a44330ad902636f1b74b4c
2 N073855c177d040bcbb677ab9bcd3b915
3 N1e65c811724147cb87eeeec3de91254f
4 N2aa7b9a8229147b2aa4248d197e6dd11
5 N2d642165cdd44087b7db51afa045dae6
6 N4d111e42d2c14baab58fdd200c9e3b78
7 N8c1555eb14fc4b00b076049a593f8021
8 N91ec38001dec4c70958e3fc9a3bf0fbf
9 Nacbeade6dac54132a027709c92257288
10 Nc987acfed9cd4f24b3c5a2e690cc8f2f
11 Ncadf2f70fb3f480e98be571ce99c48d5
12 Ncf5f929ee84c4d299bac7dda09580261
13 Ne1c7e30c34c041738a5fc2d7f4b6f3fd
14 Ne4b2faceffd24d4ca2e751e67ba22b8e
15 Nf15985253eb14261b0c1727efaccc1a9
16 anzsrc-for:06
17 anzsrc-for:0601
18 schema:author N3607d0d731a043e1bea8e5c132bf58de
19 schema:citation sg:pub.10.1007/s11248-006-9015-5
20 sg:pub.10.1007/s11248-007-9137-4
21 sg:pub.10.1007/s11248-009-9271-2
22 sg:pub.10.1023/a:1018442124584
23 sg:pub.10.1038/nbt0192-74
24 sg:pub.10.1038/nbt0397-239
25 sg:pub.10.1038/nbt0997-859
26 sg:pub.10.1038/nbt1295-1452
27 sg:pub.10.1186/1297-9686-34-1-117
28 sg:pub.10.1186/1471-2105-8-71
29 https://app.dimensions.ai/details/publication/pub.1083274025
30 https://doi.org/10.1002/(sici)1098-2795(199904)52:4<414::aid-mrd10>3.0.co;2-s
31 https://doi.org/10.1002/(sici)1098-2795(199909)54:1<17::aid-mrd3>3.0.co;2-n
32 https://doi.org/10.1002/biot.200800069
33 https://doi.org/10.1002/dvdy.22051
34 https://doi.org/10.1002/gene.10249
35 https://doi.org/10.1002/mrd.90007
36 https://doi.org/10.1006/bbrc.2000.3013
37 https://doi.org/10.1016/0092-8674(87)90584-8
38 https://doi.org/10.1016/0092-8674(93)80052-g
39 https://doi.org/10.1016/j.abb.2007.11.019
40 https://doi.org/10.1016/j.cimid.2007.11.005
41 https://doi.org/10.1016/j.cimid.2007.11.013
42 https://doi.org/10.1016/j.jbiotec.2006.10.016
43 https://doi.org/10.1016/j.jchromb.2009.04.012
44 https://doi.org/10.1016/j.molcel.2004.10.005
45 https://doi.org/10.1016/j.theriogenology.2006.09.034
46 https://doi.org/10.1016/s0003-2697(02)00273-7
47 https://doi.org/10.1016/s0070-2153(07)80009-3
48 https://doi.org/10.1016/s0163-7258(03)00069-x
49 https://doi.org/10.1017/s0022029900004386
50 https://doi.org/10.1021/bi9603783
51 https://doi.org/10.1074/jbc.275.2.759
52 https://doi.org/10.1080/10425170601017145
53 https://doi.org/10.1080/10826069308544565
54 https://doi.org/10.1089/104454903321112488
55 https://doi.org/10.1089/15362300152725936
56 https://doi.org/10.1093/glycob/10.5.477
57 https://doi.org/10.1093/glycob/cwh010
58 https://doi.org/10.1093/glycob/cwm015
59 https://doi.org/10.1093/glycob/cwn035
60 https://doi.org/10.1093/hmg/8.12.2145
61 https://doi.org/10.1093/nar/27.6.1555
62 https://doi.org/10.1101/gad.988502
63 https://doi.org/10.1101/gr.4004805
64 https://doi.org/10.1126/science.1067081
65 https://doi.org/10.1126/science.1140871
66 https://doi.org/10.1128/mcb.01326-07
67 https://doi.org/10.1128/mcb.25.6.2260-2272.2005
68 https://doi.org/10.1182/blood-2002-04-1104
69 https://doi.org/10.1371/journal.pbio.0030007
70 https://doi.org/10.1371/journal.pone.0003453
71 https://doi.org/10.1615/critreveukargeneexpr.v10.i2.60
72 https://doi.org/10.3168/jds.2008-1189
73 schema:datePublished 2012
74 schema:datePublishedReg 2012-01-01
75 schema:description Producing complex recombinant proteins in the milk of transgenic animals offers several advantages: large amounts of proteins can be obtained, and in most cases, these proteins are properly folded, assembled, cleaved, and glycosylated. The level of expression of foreign genes in the mammalian gland cannot be predicted in all cases, and appropriate vectors must be used. The main elements of these vectors are as follows: a well-characterized specific promoter, the coding region of the gene of interest, preferably with a homologous or heterologous intron, to improve transcription efficiency, and an insulator or boundary element to counteract the chromosomal position effects at the integration site. Once high expression levels are achieved, and the recombinant protein is purified, an essential step in the analysis of the final product is determining its degree of glycosylation. This is an important readout because it can affect among other parameters the stability and immunogenicity of the recombinant protein.
76 schema:editor N8305e40246db499e92714c5cf046ddee
77 schema:genre chapter
78 schema:inLanguage en
79 schema:isAccessibleForFree false
80 schema:isPartOf N00d397ffb6f54eaaa5648ca977dadfd1
81 schema:name Recombinant Protein Expression in Milk of Livestock Species
82 schema:pagination 629-41
83 schema:productId N1a4873cd56d74125a0617216c1eff4c0
84 N9355518a3f754d79b77b6ee14651c1b3
85 Nc8841b62c760428fbaa0c07b6e275a5e
86 Nd15ca240ac4344599a5f4f9bf9d88425
87 schema:publisher N3e3f1b3a95bf425497f6e8696a492992
88 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026680557
89 https://doi.org/10.1007/978-1-61779-433-9_34
90 schema:sdDatePublished 2019-04-16T00:49
91 schema:sdLicense https://scigraph.springernature.com/explorer/license/
92 schema:sdPublisher Ned05828337d54084a99fa16a9a985f6b
93 schema:url http://link.springer.com/10.1007/978-1-61779-433-9_34
94 sgo:license sg:explorer/license/
95 sgo:sdDataset chapters
96 rdf:type schema:Chapter
97 N00d397ffb6f54eaaa5648ca977dadfd1 schema:isbn 978-1-61779-432-2
98 978-1-61779-433-9
99 schema:name Recombinant Gene Expression
100 rdf:type schema:Book
101 N07182ad3a1a44330ad902636f1b74b4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Milk
103 rdf:type schema:DefinedTerm
104 N073855c177d040bcbb677ab9bcd3b915 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Microinjections
106 rdf:type schema:DefinedTerm
107 N1a4873cd56d74125a0617216c1eff4c0 schema:name dimensions_id
108 schema:value pub.1026680557
109 rdf:type schema:PropertyValue
110 N1e65c811724147cb87eeeec3de91254f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Livestock
112 rdf:type schema:DefinedTerm
113 N2aa7b9a8229147b2aa4248d197e6dd11 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Animals
115 rdf:type schema:DefinedTerm
116 N2d642165cdd44087b7db51afa045dae6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Recombinant Proteins
118 rdf:type schema:DefinedTerm
119 N3607d0d731a043e1bea8e5c132bf58de rdf:first sg:person.0600053006.69
120 rdf:rest N84fd70ef94f44cb49f326a251bb33c4e
121 N38c3128479fa4d039ebf948cd1d402cd schema:familyName Lorence
122 schema:givenName Argelia
123 rdf:type schema:Person
124 N3e3f1b3a95bf425497f6e8696a492992 schema:location Totowa, NJ
125 schema:name Humana Press
126 rdf:type schema:Organisation
127 N4d111e42d2c14baab58fdd200c9e3b78 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Genetic Vectors
129 rdf:type schema:DefinedTerm
130 N8305e40246db499e92714c5cf046ddee rdf:first N38c3128479fa4d039ebf948cd1d402cd
131 rdf:rest rdf:nil
132 N84fd70ef94f44cb49f326a251bb33c4e rdf:first sg:person.01320374062.84
133 rdf:rest rdf:nil
134 N8c1555eb14fc4b00b076049a593f8021 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Transgenes
136 rdf:type schema:DefinedTerm
137 N91ec38001dec4c70958e3fc9a3bf0fbf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Promoter Regions, Genetic
139 rdf:type schema:DefinedTerm
140 N9355518a3f754d79b77b6ee14651c1b3 schema:name pubmed_id
141 schema:value 22160924
142 rdf:type schema:PropertyValue
143 Nacbeade6dac54132a027709c92257288 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Species Specificity
145 rdf:type schema:DefinedTerm
146 Nc8841b62c760428fbaa0c07b6e275a5e schema:name readcube_id
147 schema:value 7f1a6bb4bc36589c827978df8ce15862d743c72214e1380288eb986779cf8747
148 rdf:type schema:PropertyValue
149 Nc987acfed9cd4f24b3c5a2e690cc8f2f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name DNA, Bacterial
151 rdf:type schema:DefinedTerm
152 Ncadf2f70fb3f480e98be571ce99c48d5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Chromosomes, Artificial, Bacterial
154 rdf:type schema:DefinedTerm
155 Ncf5f929ee84c4d299bac7dda09580261 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Biotechnology
157 rdf:type schema:DefinedTerm
158 Nd15ca240ac4344599a5f4f9bf9d88425 schema:name doi
159 schema:value 10.1007/978-1-61779-433-9_34
160 rdf:type schema:PropertyValue
161 Ne1c7e30c34c041738a5fc2d7f4b6f3fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Chromosomes, Artificial, Yeast
163 rdf:type schema:DefinedTerm
164 Ne4b2faceffd24d4ca2e751e67ba22b8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
166 rdf:type schema:DefinedTerm
167 Ned05828337d54084a99fa16a9a985f6b schema:name Springer Nature - SN SciGraph project
168 rdf:type schema:Organization
169 Nf15985253eb14261b0c1727efaccc1a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Animals, Genetically Modified
171 rdf:type schema:DefinedTerm
172 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
173 schema:name Biological Sciences
174 rdf:type schema:DefinedTerm
175 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
176 schema:name Biochemistry and Cell Biology
177 rdf:type schema:DefinedTerm
178 sg:person.01320374062.84 schema:affiliation https://www.grid.ac/institutes/grid.417744.5
179 schema:familyName Hiripi
180 schema:givenName László
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320374062.84
182 rdf:type schema:Person
183 sg:person.0600053006.69 schema:affiliation https://www.grid.ac/institutes/grid.417744.5
184 schema:familyName Bösze
185 schema:givenName Zsuzsanna
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600053006.69
187 rdf:type schema:Person
188 sg:pub.10.1007/s11248-006-9015-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003563056
189 https://doi.org/10.1007/s11248-006-9015-5
190 rdf:type schema:CreativeWork
191 sg:pub.10.1007/s11248-007-9137-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022392937
192 https://doi.org/10.1007/s11248-007-9137-4
193 rdf:type schema:CreativeWork
194 sg:pub.10.1007/s11248-009-9271-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052514860
195 https://doi.org/10.1007/s11248-009-9271-2
196 rdf:type schema:CreativeWork
197 sg:pub.10.1023/a:1018442124584 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005524006
198 https://doi.org/10.1023/a:1018442124584
199 rdf:type schema:CreativeWork
200 sg:pub.10.1038/nbt0192-74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043711637
201 https://doi.org/10.1038/nbt0192-74
202 rdf:type schema:CreativeWork
203 sg:pub.10.1038/nbt0397-239 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022614305
204 https://doi.org/10.1038/nbt0397-239
205 rdf:type schema:CreativeWork
206 sg:pub.10.1038/nbt0997-859 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031958037
207 https://doi.org/10.1038/nbt0997-859
208 rdf:type schema:CreativeWork
209 sg:pub.10.1038/nbt1295-1452 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011850752
210 https://doi.org/10.1038/nbt1295-1452
211 rdf:type schema:CreativeWork
212 sg:pub.10.1186/1297-9686-34-1-117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022879721
213 https://doi.org/10.1186/1297-9686-34-1-117
214 rdf:type schema:CreativeWork
215 sg:pub.10.1186/1471-2105-8-71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014726920
216 https://doi.org/10.1186/1471-2105-8-71
217 rdf:type schema:CreativeWork
218 https://app.dimensions.ai/details/publication/pub.1083274025 schema:CreativeWork
219 https://doi.org/10.1002/(sici)1098-2795(199904)52:4<414::aid-mrd10>3.0.co;2-s schema:sameAs https://app.dimensions.ai/details/publication/pub.1038575760
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1002/(sici)1098-2795(199909)54:1<17::aid-mrd3>3.0.co;2-n schema:sameAs https://app.dimensions.ai/details/publication/pub.1003306980
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1002/biot.200800069 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019425468
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1002/dvdy.22051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031623864
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1002/gene.10249 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008837647
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1002/mrd.90007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015847382
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1006/bbrc.2000.3013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025375587
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/0092-8674(87)90584-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041471577
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/0092-8674(93)80052-g schema:sameAs https://app.dimensions.ai/details/publication/pub.1030579809
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/j.abb.2007.11.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036163300
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/j.cimid.2007.11.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041897979
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/j.cimid.2007.11.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021178827
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/j.jbiotec.2006.10.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035592352
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/j.jchromb.2009.04.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010130547
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/j.molcel.2004.10.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043611646
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/j.theriogenology.2006.09.034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042529548
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1016/s0003-2697(02)00273-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009026616
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1016/s0070-2153(07)80009-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047834280
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1016/s0163-7258(03)00069-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040880042
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1017/s0022029900004386 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054049010
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1021/bi9603783 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055211762
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1074/jbc.275.2.759 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053656874
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1080/10425170601017145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006597680
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1080/10826069308544565 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026487676
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1089/104454903321112488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059203798
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1089/15362300152725936 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059214841
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1093/glycob/10.5.477 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001266444
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1093/glycob/cwh010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014083759
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1093/glycob/cwm015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011623040
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1093/glycob/cwn035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049370417
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1093/hmg/8.12.2145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027096024
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1093/nar/27.6.1555 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048868488
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1101/gad.988502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007648644
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1101/gr.4004805 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060407529
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1126/science.1067081 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018333078
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1126/science.1140871 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062455912
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1128/mcb.01326-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052357308
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1128/mcb.25.6.2260-2272.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027050792
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1182/blood-2002-04-1104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021476120
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1371/journal.pbio.0030007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039517858
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1371/journal.pone.0003453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031242823
300 rdf:type schema:CreativeWork
301 https://doi.org/10.1615/critreveukargeneexpr.v10.i2.60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068130907
302 rdf:type schema:CreativeWork
303 https://doi.org/10.3168/jds.2008-1189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077815204
304 rdf:type schema:CreativeWork
305 https://www.grid.ac/institutes/grid.417744.5 schema:alternateName Agricultural Biotechnology Institute
306 schema:name Agricultural Biotechnology Center, Gödöll, Hungary
307 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...