Recombinant Protein Expression in Milk of Livestock Species View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Zsuzsanna Bösze , László Hiripi

ABSTRACT

Producing complex recombinant proteins in the milk of transgenic animals offers several advantages: large amounts of proteins can be obtained, and in most cases, these proteins are properly folded, assembled, cleaved, and glycosylated. The level of expression of foreign genes in the mammalian gland cannot be predicted in all cases, and appropriate vectors must be used. The main elements of these vectors are as follows: a well-characterized specific promoter, the coding region of the gene of interest, preferably with a homologous or heterologous intron, to improve transcription efficiency, and an insulator or boundary element to counteract the chromosomal position effects at the integration site. Once high expression levels are achieved, and the recombinant protein is purified, an essential step in the analysis of the final product is determining its degree of glycosylation. This is an important readout because it can affect among other parameters the stability and immunogenicity of the recombinant protein. More... »

PAGES

629-41

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-433-9_34

DOI

http://dx.doi.org/10.1007/978-1-61779-433-9_34

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026680557

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22160924


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RDF/XML is a standard XML format for linked data.

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