In Vitro Generation of Megakaryocytes and Platelets from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2011-11-17

AUTHORS

Naoya Takayama , Koji Eto

ABSTRACT

Human embryonic stem cells (hESCs) represent a potential source of blood cells for transfusion therapies and a promising tool for studying the ontogeny of hematopoiesis. Moreover, human-induced pluripotent stem cells (hiPSCs), recently established by defined reprogramming factors expressed in somatic cells, represent a further source for the generation of hematopoietic cells. When undifferentiated hESCs or hiPSCs are cultured on either mesenchymal C3H10T1/2 cells or OP-9 stromal cells, they can be differentiated into a hematopoietic niche that concentrates hematopoietic progenitors, which we named “embryonic stem cell-derived sacs” (ES-sacs). We have optimized the in vitro culture condition for obtaining mature megakaryocytes derived from the hematopoietic progenitors within ES-sacs, which are then able to release platelets. These in vitro-generated platelets display integrin activation capability, indicating normal hemostatic function. This novel protocol thus provides a means of generating platelets from hESCs as well as hiPSCs, for the study of normal human thrombopoiesis and also thrombopoiesis in disease conditions using patient-specific hiPSCs. More... »

PAGES

205-217

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-307-3_15

DOI

http://dx.doi.org/10.1007/978-1-61779-307-3_15

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010375591

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22130710


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