Simultaneous Observation of Chemomechanical Coupling of a Molecular Motor View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2011-07-01

AUTHORS

Takayuki Nishizaka , Yuh Hasimoto , Tomoko Masaike

ABSTRACT

F1-ATPase is the smallest rotary molecular motor ever found. Unidirectional rotation of the γ-shaft is driven by precisely coordinated sequential ATP hydrolysis reactions in three catalytic sites arranged 120° apart in the cylinder. Single-molecule observation allows us to directly watch the rotation of the shaft using micron-sized plastic beads. Additionally, an advanced version of “total internal reflection fluorescence microscope (TIRFM)” enables us to detect binding and release of energy currency through fluorescently labeled ATP. In this chapter, we describe how to set up the system for simultaneous observation of these two critical events. This specialized optical setup is applicable to a variety of research, not only molecular motors but also other single-molecule topics. More... »

PAGES

259-271

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-261-8_17

DOI

http://dx.doi.org/10.1007/978-1-61779-261-8_17

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014069643

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21809212


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