In Silico Methods for Identifying Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of ... View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2011-07-02

AUTHORS

Sandra K. Tanz , Ian Small

ABSTRACT

Numerous experimental and in silico approaches have been developed for attempting to identify the -subcellular localisation of proteins. Approximately 2,000-4,000 proteins are thought to be targeted to plastids in plants, but a complete and unambiguous catalogue has yet to be drawn up. This article reviews the various prediction methods that identify plastid targeting sequences, and those that can help estimate location and topology within the plastid or plastid membranes. The most successful approaches are described in detail, with detailed notes to help avoid common pitfalls and advice on interpreting conflicting or ambiguous results. In most cases, it is best to try multiple approaches, and we also cover the powerful new integrated databases that provide a selected blend of experimental data and predictions. More... »

PAGES

243-280

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-234-2_16

DOI

http://dx.doi.org/10.1007/978-1-61779-234-2_16

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018964775

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21822844


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