Culture Adaptation of Pluripotent Stem Cells: Challenges and Opportunities View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2011-07-22

AUTHORS

Neil J. Harrison , Duncan Baker , Peter W. Andrews

ABSTRACT

Embryonic stem (ES) cells may acquire genetic and epigenetic changes upon prolonged passage in culture, which can confer on them more robust growth characteristics. The genetic changes are often manifest cytogenetically as nonrandom gains of chromosomal regions that are also typically amplified in embryonal carcinoma (EC) cells, the malignant counterpart of ES cells. Although this raises some concerns for the future use of ES or induced pluripotent stem (iPS) cells in regenerative medicine, or in vitro screening applications, these concerns remain largely hypothetical. It may be that potential problems can be substantially ­mitigated when we understand more about the underlying causes and mechanisms of culture adaptation. At the same time, this phenomenon also provides a tool that can help dissect the mechanisms controlling stem cell behavior, while potentially providing more robust cells for use in some applications. More... »

PAGES

265-276

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  • Book

    TITLE

    Nuclear Reprogramming and Stem Cells

    ISBN

    978-1-61779-224-3
    978-1-61779-225-0

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-61779-225-0_19

    DOI

    http://dx.doi.org/10.1007/978-1-61779-225-0_19

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014308728


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