A Transposon-Based Activation Tagging System for Gene Function Discovery in Arabidopsis View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2011-06-22

AUTHORS

Nayelli Marsch-Martínez

ABSTRACT

Activation tagging is a powerful strategy to find new gene functions, especially from genes that are redundant or show lethal knock-out phenotypes. It has been applied using T-DNA or transposons. En/Spm-I/dSpm engineered transposons are efficient activation tags in Arabidopsis. An immobilized transposase source and an enhancer-bearing non-autonomous element are used in combination with positive and negative selectable markers to generate a population of single- or low-copy, stable insertions. This method describes the steps required for selection of parental lines, generation of a population of stable insertions, and gene identification. More... »

PAGES

67-83

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61779-154-3_4

DOI

http://dx.doi.org/10.1007/978-1-61779-154-3_4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050294161

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21720947


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