Fluorescent Protein-Assisted Purification for Gene Expression Profiling View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2010-11-15

AUTHORS

M. Raza Zaidi , Chi-Ping Day , Glenn Merlino

ABSTRACT

Cell type-specific expression of fluorescent proteins allows the purification of rare cells from complex ­tissues by flow cytometry. This strategy is especially useful for molecular analysis of cancer cells because these cells can be effectively purified away from the noncancerous tumor stroma. Coexpression of bioluminescence with fluorescence makes further allows in vivo tracking of cancer cells, which can then be purified at specific tumorigenic stages. Here, we describe protocols for purifying rare skin stem cells, and for in vivo monitoring and purification of cancer cells from lung metastases. Also described is a protocol for the isolation of total RNA from the purified cells for the purpose of performing gene expression profiling. More... »

PAGES

393-405

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-61737-950-5_19

DOI

http://dx.doi.org/10.1007/978-1-61737-950-5_19

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035623003

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21116994


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