In Situ Chemical Modification of Peptide Microarrays: Application to the Study of the Antibody Responses to Methylated Antigens View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2010-08-19

AUTHORS

Rémi Desmet , Eric Diesis , Hervé Drobecq , Carine Rouanet , Karim Chemlal , Anne-Sophie Debrie , Jean-Michel Hougardy , Françoise Mascart , Camille Locht , Oleg Melnyk

ABSTRACT

Peptide microarrays are useful tools for characterizing the humoral response against methylated antigens. They are usually prepared by printing unmodified and methylated peptides on substrates such as functionalized microscope glass slides. The preferential capture of antibodies by methylated peptides suggests the specific recognition of methylated epitopes. However, unmodified peptide epitopes can be masked due to their interaction with the substrate. The accessibility of unmodified peptides and thus the specificity of the recognition of methylated peptide epitopes can be probed using the in situ methylation procedure described here. Alternately, the in situ methylation of peptide microarrays allows probing the presence of antibodies directed toward methylated epitopes starting from easy-to-make and cost-effective unmodified peptide libraries. In situ methylation was performed using formaldehyde in the presence of sodium cyanoborohydride and nickel chloride. This chemical procedure converts lysine residues into mono- or dimethyl lysines. More... »

PAGES

135-145

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60761-845-4_11

DOI

http://dx.doi.org/10.1007/978-1-60761-845-4_11

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044959923

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20857363


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-60761-845-4_11'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-60761-845-4_11'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-60761-845-4_11'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-60761-845-4_11'


 

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312 https://www.grid.ac/institutes/grid.8970.6 schema:alternateName Pasteur Institute of Lille
313 schema:name IFR 142 Molecular and Cellular Medicine, Lille, France
314 INSERM U1019, Center for Infection and Immunity, CNRS UMR8204, IFR 142 Molecular and Cellular Medicine, Lille, France
315 INSERM U629, Institut Pasteur de Lille, Lille, France
316 Institut Pasteur de Lille, Lille, France
317 UMR 8161 CNRS-Université de Lille Nord de France, Lille, France
318 rdf:type schema:Organization
 




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