Forward RNAi Screens in Human Stem Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2010-05-31

AUTHORS

Christine Karlsson , Jonas Larsson , Aurélie Baudet

ABSTRACT

Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells. These cells are suitable targets for complex, selection-based screens using pooled lentiviral shRNA libraries. The screening approach is a promising new tool to dissect regulatory mechanisms in hematopoietic and somatic stem cells, in general, and may be particularly useful to identify gene targets and modifiers that can be further exploited in strategies for ex vivo stem cell expansion. More... »

PAGES

29-43

Book

TITLE

RNAi and microRNA-Mediated Gene Regulation in Stem Cells

ISBN

978-1-60761-768-6
978-1-60761-769-3

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60761-769-3_3

DOI

http://dx.doi.org/10.1007/978-1-60761-769-3_3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042817287

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20686941


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