Preparation and Analysis of MicroRNA Libraries Using the Illumina Massively Parallel Sequencing Technology View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2010-05-31

AUTHORS

Ryan D. Morin , Yongjun Zhao , Anna-Liisa Prabhu , Noreen Dhalla , Helen McDonald , Pawan Pandoh , Angela Tam , Thomas Zeng , Martin Hirst , Marco Marra

ABSTRACT

MicroRNAs are key regulators of gene expression in diverse biological processes and their importance in embryonic stem cells is indisputable. New 'next-generation' technologies such as Illumina massively parallel sequencing offer vast improvements, in both scale and sensitivity, to microRNA profiling studies. We describe a detailed procedure for the preparation of small RNA libraries for Illumina sequencing. We further comment on approaches for analyzing the resultant sequence data for measuring microRNA abundance. More... »

PAGES

173-199

Book

TITLE

RNAi and microRNA-Mediated Gene Regulation in Stem Cells

ISBN

978-1-60761-768-6
978-1-60761-769-3

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60761-769-3_14

DOI

http://dx.doi.org/10.1007/978-1-60761-769-3_14

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031022682

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20686952


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