Designing Optimal siRNA Based on Target Site Accessibility View Full Text


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Chapter Info

DATE

2010-01-21

AUTHORS

Ivo L. Hofacker , Hakim Tafer

ABSTRACT

RNA interference, mediated by small interfering RNAs (siRNAs), is a powerful tool for investigation of gene functions and it is increasingly being used as a therapeutic agent. However, not all siRNAs are equally potent - although simple rules for the selection of good siRNAs were proposed early on, siRNAs are still plagued with widely fluctuating efficiency. Recently, new design tools that incorporate both the structural features of the targeted RNAs and the sequence features of the siRNAs have substantially improved the efficacy of siRNAs. In this chapter, we present the algorithms behind these accessibility-aided tools and show how to design efficient siRNAs with their help. More... »

PAGES

137-154

References to SciGraph publications

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  • Book

    TITLE

    RNA Interference

    ISBN

    978-1-60761-587-3
    978-1-60761-588-0

    Author Affiliations

    From Grant

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-60761-588-0_9

    DOI

    http://dx.doi.org/10.1007/978-1-60761-588-0_9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1002148027

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20217549


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