An Overview of Label-Free Quantitation Methods in Proteomics by Mass Spectrometry View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009-12-05

AUTHORS

Jason W. H. Wong , Gerard Cagney

ABSTRACT

Protein quantification represents an important extension to identification proteomics, enabling the comparison of protein expression across different samples or treatments. Comparative protein quantification by mass spectrometry typically employs stable isotope incorporation, but recently, comparative quantification of label-free LCn-MS proteomics data has emerged as an alternative approach. In this chapter, we provide an overview of the different approaches for extracting quantitative data from label-free LCn-MS experiments. The computational procedure for recovering the quantitative information is outlined. Examples of statistical tests used to evaluate the relevance of results are also provided. More... »

PAGES

273-283

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60761-444-9_18

DOI

http://dx.doi.org/10.1007/978-1-60761-444-9_18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013578040

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20013377


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