Combination of Fluorescence In Situ Hybridization with Staining Techniques for Cell Viability and Accumulation of PHA and polyP in Microorganisms ... View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009-10-25

AUTHORS

Jeppe Lund Nielsen , Caroline Kragelund , Per Halkjær Nielsen

ABSTRACT

Fluorescence in situ hybridization (FISH) can be combined with a number of staining techniques to reveal the relationships between the microorganisms and their function in complex microbial systems with a single-cell resolution. In this chapter, we have focused on staining methods for intracellular storage compounds (polyhydroxyalkanoates, polyphosphate) and a measure for cell viability, reduction of the tetrazolium-based redox stain CTC. These protocols are optimized for the study of microorganisms in waste-water treatment (activated sludge and biofilms), but they may also be used with minor modifications in many other ecosystems. More... »

PAGES

103-116

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60761-439-5_7

DOI

http://dx.doi.org/10.1007/978-1-60761-439-5_7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001034049

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19882282


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