Using RNA FISH to Study Gene Expression During Mammalian Meiosis View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009-06-06

AUTHORS

Shantha K. Mahadevaiah , Yael Costa , James M. A. Turner

ABSTRACT

During mouse meiosis, gene expression and homologous synapsis are intimately linked. Chromosomes that fail to synapse at the zygotene-pachytene transition become transcriptionally silenced by a process called Meiotic Silencing of Unsynapsed Chromatin (MSUC), and this silencing (or defects in it) may in turn cause germ cell losses and infertility. Gene transcription at the chromosomal level can be readily observed using RNA fluorescence in-situ hybridisation (FISH), and this approach allows one to directly compare expression at a specific locus with the synaptic status of the chromosome domain on which it resides. Here we describe a protocol for carrying out RNA FISH on male meiotic cells, together with detail on the important controls and common problems associated with this technique. More... »

PAGES

433-444

References to SciGraph publications

  • 2005-01. Synapsis or silence in NATURE GENETICS
  • 2005-01. Silencing of unsynapsed meiotic chromosomes in the mouse in NATURE GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-60761-103-5_25

    DOI

    http://dx.doi.org/10.1007/978-1-60761-103-5_25

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19685339


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