Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009

AUTHORS

Marco Geymonat , Adonis Spanos , Steven Sedgwick

ABSTRACT

A novel protein expression system in budding yeast is described which has been used to express many yeast mitotic regulators as well as a wide range of other recombinant proteins from several different species. The expression system relies on autoselection with essential genes to maintain high copy numbers of expression plasmids. Autoselection permits expression cells to be grown in rich medium with no need for plasmid selection with drugs or nutritional conditions. This optimizes growth and expression of recombinant proteins. The use of the expression system is illustrated by purifying budding yeast mitotic regulators, Cdc14 and Net1, and recapitulating their activities in vitro. More... »

PAGES

63-80

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60327-993-2_4

DOI

http://dx.doi.org/10.1007/978-1-60327-993-2_4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036737590

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19475382


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