Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009

AUTHORS

Marco Geymonat , Adonis Spanos , Steven Sedgwick

ABSTRACT

A novel protein expression system in budding yeast is described which has been used to express many yeast mitotic regulators as well as a wide range of other recombinant proteins from several different species. The expression system relies on autoselection with essential genes to maintain high copy numbers of expression plasmids. Autoselection permits expression cells to be grown in rich medium with no need for plasmid selection with drugs or nutritional conditions. This optimizes growth and expression of recombinant proteins. The use of the expression system is illustrated by purifying budding yeast mitotic regulators, Cdc14 and Net1, and recapitulating their activities in vitro. More... »

PAGES

63-80

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60327-993-2_4

DOI

http://dx.doi.org/10.1007/978-1-60327-993-2_4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036737590

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19475382


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Tyrosine Phosphatases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "Stem Cell Biology and Developmental Genetics, National Institute for Medical Research, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Geymonat", 
        "givenName": "Marco", 
        "id": "sg:person.0653577454.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653577454.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "Stem Cell Biology and Developmental Genetics, National Institute for Medical Research, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Spanos", 
        "givenName": "Adonis", 
        "id": "sg:person.010107334027.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010107334027.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "Stem Cell Biology and Developmental Genetics, National Institute for Medical Research, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sedgwick", 
        "givenName": "Steven", 
        "id": "sg:person.015016201267.68", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015016201267.68"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/19775", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003128483", 
          "https://doi.org/10.1038/19775"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/19775", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003128483", 
          "https://doi.org/10.1038/19775"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m011689200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025589174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.gene.2007.05.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030354208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80733-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043251016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2005.04.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052815216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082809367", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009", 
    "datePublishedReg": "2009-01-01", 
    "description": "A novel protein expression system in budding yeast is described which has been used to express many yeast mitotic regulators as well as a wide range of other recombinant proteins from several different species. The expression system relies on autoselection with essential genes to maintain high copy numbers of expression plasmids. Autoselection permits expression cells to be grown in rich medium with no need for plasmid selection with drugs or nutritional conditions. This optimizes growth and expression of recombinant proteins. The use of the expression system is illustrated by purifying budding yeast mitotic regulators, Cdc14 and Net1, and recapitulating their activities in vitro.", 
    "editor": [
      {
        "familyName": "McAinsh", 
        "givenName": "Andrew D.", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-60327-993-2_4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-60327-992-5", 
        "978-1-60327-993-2"
      ], 
      "name": "Mitosis", 
      "type": "Book"
    }, 
    "name": "Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast", 
    "pagination": "63-80", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-60327-993-2_4"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "af00eaf1505f7ec499b44d45c35c71b39fb432d57e575e1547286542c5e6a3d3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036737590"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19475382"
        ]
      }
    ], 
    "publisher": {
      "location": "Totowa, NJ", 
      "name": "Humana Press", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-60327-993-2_4", 
      "https://app.dimensions.ai/details/publication/pub.1036737590"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T10:36", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000266.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-60327-993-2_4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-993-2_4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-993-2_4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-993-2_4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-993-2_4'


 

This table displays all metadata directly associated to this object as RDF triples.

137 TRIPLES      23 PREDICATES      43 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-60327-993-2_4 schema:about N42734d6ebad74e9090f7ee6f6c6aa9a1
2 N60d23e8f8c6747f7890c24ac5fcc1985
3 N7d3731a4c7e94e71ac2d808eda0eb612
4 N97f04d0d3e9748e1a1d2187c6c2f3449
5 N9cb3905ba3c24f2cb87ad74929ee1629
6 Na205d90c07d6454ea8e1da2a97202f4f
7 Na66253e732b04f17b161b0bd430e332b
8 Nb599c9a259af4d40a269dc81aa9c99aa
9 Nf4b9199c7db745e99c7ac5974603f0a3
10 anzsrc-for:06
11 anzsrc-for:0601
12 schema:author N645be9388a3c4de896ae887487b86b7a
13 schema:citation sg:pub.10.1038/19775
14 https://app.dimensions.ai/details/publication/pub.1082809367
15 https://doi.org/10.1016/j.cell.2005.04.006
16 https://doi.org/10.1016/j.gene.2007.05.001
17 https://doi.org/10.1016/s0092-8674(00)80733-3
18 https://doi.org/10.1074/jbc.m011689200
19 schema:datePublished 2009
20 schema:datePublishedReg 2009-01-01
21 schema:description A novel protein expression system in budding yeast is described which has been used to express many yeast mitotic regulators as well as a wide range of other recombinant proteins from several different species. The expression system relies on autoselection with essential genes to maintain high copy numbers of expression plasmids. Autoselection permits expression cells to be grown in rich medium with no need for plasmid selection with drugs or nutritional conditions. This optimizes growth and expression of recombinant proteins. The use of the expression system is illustrated by purifying budding yeast mitotic regulators, Cdc14 and Net1, and recapitulating their activities in vitro.
22 schema:editor Ne11f89b671364220a5aa7467a77c802e
23 schema:genre chapter
24 schema:inLanguage en
25 schema:isAccessibleForFree false
26 schema:isPartOf N5d652f3772e34a6ba1b48c3e61d475af
27 schema:name Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast
28 schema:pagination 63-80
29 schema:productId N3aa7e94713324c7ba6d7cfaf2a12ae15
30 N98cd49fb756149c9b0d084ece725b8b9
31 Nc6ccb800e3a84e3fa02344bd0375c2e1
32 Ncd020ace089748828981958380eb2228
33 schema:publisher N2430310d4fb14e01834acfdcc15d5dbd
34 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036737590
35 https://doi.org/10.1007/978-1-60327-993-2_4
36 schema:sdDatePublished 2019-04-15T10:36
37 schema:sdLicense https://scigraph.springernature.com/explorer/license/
38 schema:sdPublisher N9e0705bf762643ad9cd08fd987d68485
39 schema:url http://link.springer.com/10.1007/978-1-60327-993-2_4
40 sgo:license sg:explorer/license/
41 sgo:sdDataset chapters
42 rdf:type schema:Chapter
43 N2430310d4fb14e01834acfdcc15d5dbd schema:location Totowa, NJ
44 schema:name Humana Press
45 rdf:type schema:Organisation
46 N3aa7e94713324c7ba6d7cfaf2a12ae15 schema:name pubmed_id
47 schema:value 19475382
48 rdf:type schema:PropertyValue
49 N42734d6ebad74e9090f7ee6f6c6aa9a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
50 schema:name Saccharomyces cerevisiae
51 rdf:type schema:DefinedTerm
52 N5d652f3772e34a6ba1b48c3e61d475af schema:isbn 978-1-60327-992-5
53 978-1-60327-993-2
54 schema:name Mitosis
55 rdf:type schema:Book
56 N60d23e8f8c6747f7890c24ac5fcc1985 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
57 schema:name Base Sequence
58 rdf:type schema:DefinedTerm
59 N645be9388a3c4de896ae887487b86b7a rdf:first sg:person.0653577454.35
60 rdf:rest N8e36343754de4deca516626b99af1e88
61 N7d3731a4c7e94e71ac2d808eda0eb612 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Molecular Sequence Data
63 rdf:type schema:DefinedTerm
64 N8a72724e24164a31b39e91733086d960 schema:familyName McAinsh
65 schema:givenName Andrew D.
66 rdf:type schema:Person
67 N8e36343754de4deca516626b99af1e88 rdf:first sg:person.010107334027.29
68 rdf:rest Nc205510d693e4a878b8fd025a2b36e4b
69 N97f04d0d3e9748e1a1d2187c6c2f3449 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Plasmids
71 rdf:type schema:DefinedTerm
72 N98cd49fb756149c9b0d084ece725b8b9 schema:name dimensions_id
73 schema:value pub.1036737590
74 rdf:type schema:PropertyValue
75 N9cb3905ba3c24f2cb87ad74929ee1629 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Cell Cycle Proteins
77 rdf:type schema:DefinedTerm
78 N9e0705bf762643ad9cd08fd987d68485 schema:name Springer Nature - SN SciGraph project
79 rdf:type schema:Organization
80 Na205d90c07d6454ea8e1da2a97202f4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Protein Tyrosine Phosphatases
82 rdf:type schema:DefinedTerm
83 Na66253e732b04f17b161b0bd430e332b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Amino Acid Sequence
85 rdf:type schema:DefinedTerm
86 Nb599c9a259af4d40a269dc81aa9c99aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Nuclear Proteins
88 rdf:type schema:DefinedTerm
89 Nc205510d693e4a878b8fd025a2b36e4b rdf:first sg:person.015016201267.68
90 rdf:rest rdf:nil
91 Nc6ccb800e3a84e3fa02344bd0375c2e1 schema:name readcube_id
92 schema:value af00eaf1505f7ec499b44d45c35c71b39fb432d57e575e1547286542c5e6a3d3
93 rdf:type schema:PropertyValue
94 Ncd020ace089748828981958380eb2228 schema:name doi
95 schema:value 10.1007/978-1-60327-993-2_4
96 rdf:type schema:PropertyValue
97 Ne11f89b671364220a5aa7467a77c802e rdf:first N8a72724e24164a31b39e91733086d960
98 rdf:rest rdf:nil
99 Nf4b9199c7db745e99c7ac5974603f0a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Saccharomyces cerevisiae Proteins
101 rdf:type schema:DefinedTerm
102 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
103 schema:name Biological Sciences
104 rdf:type schema:DefinedTerm
105 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
106 schema:name Biochemistry and Cell Biology
107 rdf:type schema:DefinedTerm
108 sg:person.010107334027.29 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
109 schema:familyName Spanos
110 schema:givenName Adonis
111 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010107334027.29
112 rdf:type schema:Person
113 sg:person.015016201267.68 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
114 schema:familyName Sedgwick
115 schema:givenName Steven
116 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015016201267.68
117 rdf:type schema:Person
118 sg:person.0653577454.35 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
119 schema:familyName Geymonat
120 schema:givenName Marco
121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653577454.35
122 rdf:type schema:Person
123 sg:pub.10.1038/19775 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003128483
124 https://doi.org/10.1038/19775
125 rdf:type schema:CreativeWork
126 https://app.dimensions.ai/details/publication/pub.1082809367 schema:CreativeWork
127 https://doi.org/10.1016/j.cell.2005.04.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052815216
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1016/j.gene.2007.05.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030354208
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1016/s0092-8674(00)80733-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043251016
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1074/jbc.m011689200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025589174
134 rdf:type schema:CreativeWork
135 https://www.grid.ac/institutes/grid.451388.3 schema:alternateName The Francis Crick Institute
136 schema:name Stem Cell Biology and Developmental Genetics, National Institute for Medical Research, London, UK
137 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...