Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009

AUTHORS

Marco Geymonat , Adonis Spanos , Steven Sedgwick

ABSTRACT

A novel protein expression system in budding yeast is described which has been used to express many yeast mitotic regulators as well as a wide range of other recombinant proteins from several different species. The expression system relies on autoselection with essential genes to maintain high copy numbers of expression plasmids. Autoselection permits expression cells to be grown in rich medium with no need for plasmid selection with drugs or nutritional conditions. This optimizes growth and expression of recombinant proteins. The use of the expression system is illustrated by purifying budding yeast mitotic regulators, Cdc14 and Net1, and recapitulating their activities in vitro. More... »

PAGES

63-80

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60327-993-2_4

DOI

http://dx.doi.org/10.1007/978-1-60327-993-2_4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036737590

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19475382


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Tyrosine Phosphatases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "Stem Cell Biology and Developmental Genetics, National Institute for Medical Research, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Geymonat", 
        "givenName": "Marco", 
        "id": "sg:person.0653577454.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653577454.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "Stem Cell Biology and Developmental Genetics, National Institute for Medical Research, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Spanos", 
        "givenName": "Adonis", 
        "id": "sg:person.010107334027.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010107334027.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "Stem Cell Biology and Developmental Genetics, National Institute for Medical Research, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sedgwick", 
        "givenName": "Steven", 
        "id": "sg:person.015016201267.68", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015016201267.68"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/19775", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003128483", 
          "https://doi.org/10.1038/19775"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/19775", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003128483", 
          "https://doi.org/10.1038/19775"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m011689200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025589174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.gene.2007.05.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030354208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80733-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043251016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2005.04.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052815216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082809367", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009", 
    "datePublishedReg": "2009-01-01", 
    "description": "A novel protein expression system in budding yeast is described which has been used to express many yeast mitotic regulators as well as a wide range of other recombinant proteins from several different species. The expression system relies on autoselection with essential genes to maintain high copy numbers of expression plasmids. Autoselection permits expression cells to be grown in rich medium with no need for plasmid selection with drugs or nutritional conditions. This optimizes growth and expression of recombinant proteins. The use of the expression system is illustrated by purifying budding yeast mitotic regulators, Cdc14 and Net1, and recapitulating their activities in vitro.", 
    "editor": [
      {
        "familyName": "McAinsh", 
        "givenName": "Andrew D.", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-60327-993-2_4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-60327-992-5", 
        "978-1-60327-993-2"
      ], 
      "name": "Mitosis", 
      "type": "Book"
    }, 
    "name": "Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast", 
    "pagination": "63-80", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-60327-993-2_4"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "af00eaf1505f7ec499b44d45c35c71b39fb432d57e575e1547286542c5e6a3d3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036737590"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19475382"
        ]
      }
    ], 
    "publisher": {
      "location": "Totowa, NJ", 
      "name": "Humana Press", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-60327-993-2_4", 
      "https://app.dimensions.ai/details/publication/pub.1036737590"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T10:36", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000266.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-60327-993-2_4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-993-2_4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-993-2_4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-993-2_4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-993-2_4'


 

This table displays all metadata directly associated to this object as RDF triples.

137 TRIPLES      23 PREDICATES      43 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-60327-993-2_4 schema:about N070a4e2ad5c04333ad4316d3223f2dbd
2 N1bcc414186ba462c8724e20290daae1a
3 N490f5bd66583413792d631ffb18b9214
4 N4b6f8b122ac14956adec83f3bc50b2b6
5 N66a806c2721f40499e78260af6f050ea
6 N6c085937f7374e91aabe6a46783a3f25
7 Nb72c4f1c94f043929337b7a465edf500
8 Nd99752e3d635442e91525e1ee074584e
9 Nfd0767e2e8684470a9e30fd0dcefbafe
10 anzsrc-for:06
11 anzsrc-for:0601
12 schema:author Naae459868d674c28a2b8e7a0fa9fb638
13 schema:citation sg:pub.10.1038/19775
14 https://app.dimensions.ai/details/publication/pub.1082809367
15 https://doi.org/10.1016/j.cell.2005.04.006
16 https://doi.org/10.1016/j.gene.2007.05.001
17 https://doi.org/10.1016/s0092-8674(00)80733-3
18 https://doi.org/10.1074/jbc.m011689200
19 schema:datePublished 2009
20 schema:datePublishedReg 2009-01-01
21 schema:description A novel protein expression system in budding yeast is described which has been used to express many yeast mitotic regulators as well as a wide range of other recombinant proteins from several different species. The expression system relies on autoselection with essential genes to maintain high copy numbers of expression plasmids. Autoselection permits expression cells to be grown in rich medium with no need for plasmid selection with drugs or nutritional conditions. This optimizes growth and expression of recombinant proteins. The use of the expression system is illustrated by purifying budding yeast mitotic regulators, Cdc14 and Net1, and recapitulating their activities in vitro.
22 schema:editor Nc97f3daf4a6f489f89d46d40bb7f3626
23 schema:genre chapter
24 schema:inLanguage en
25 schema:isAccessibleForFree false
26 schema:isPartOf N17cc7ced7e02462daf843e4005957b12
27 schema:name Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast
28 schema:pagination 63-80
29 schema:productId N239b495459e2416a92923e65597b42b1
30 N74dd17e7f5e145b0baaa72e51d104d21
31 N9e631e69cd41452c8b4248c254aa0a25
32 Nb87fce91790f4cceb67fa8039792e2f4
33 schema:publisher N3febbb34f70e4c04b8b71905a9d5a8b5
34 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036737590
35 https://doi.org/10.1007/978-1-60327-993-2_4
36 schema:sdDatePublished 2019-04-15T10:36
37 schema:sdLicense https://scigraph.springernature.com/explorer/license/
38 schema:sdPublisher N3edbea02bd1946079a55c209d76ef97d
39 schema:url http://link.springer.com/10.1007/978-1-60327-993-2_4
40 sgo:license sg:explorer/license/
41 sgo:sdDataset chapters
42 rdf:type schema:Chapter
43 N0468f6d34e774a1d9cd3c1747faccb37 rdf:first sg:person.010107334027.29
44 rdf:rest N2e97d70b4d0c4ff597625556e882d50b
45 N070a4e2ad5c04333ad4316d3223f2dbd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
46 schema:name Base Sequence
47 rdf:type schema:DefinedTerm
48 N17cc7ced7e02462daf843e4005957b12 schema:isbn 978-1-60327-992-5
49 978-1-60327-993-2
50 schema:name Mitosis
51 rdf:type schema:Book
52 N1bcc414186ba462c8724e20290daae1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
53 schema:name Molecular Sequence Data
54 rdf:type schema:DefinedTerm
55 N239b495459e2416a92923e65597b42b1 schema:name pubmed_id
56 schema:value 19475382
57 rdf:type schema:PropertyValue
58 N2e97d70b4d0c4ff597625556e882d50b rdf:first sg:person.015016201267.68
59 rdf:rest rdf:nil
60 N3edbea02bd1946079a55c209d76ef97d schema:name Springer Nature - SN SciGraph project
61 rdf:type schema:Organization
62 N3febbb34f70e4c04b8b71905a9d5a8b5 schema:location Totowa, NJ
63 schema:name Humana Press
64 rdf:type schema:Organisation
65 N490f5bd66583413792d631ffb18b9214 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Cell Cycle Proteins
67 rdf:type schema:DefinedTerm
68 N4b6f8b122ac14956adec83f3bc50b2b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Amino Acid Sequence
70 rdf:type schema:DefinedTerm
71 N66a806c2721f40499e78260af6f050ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Protein Tyrosine Phosphatases
73 rdf:type schema:DefinedTerm
74 N6c085937f7374e91aabe6a46783a3f25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Nuclear Proteins
76 rdf:type schema:DefinedTerm
77 N74dd17e7f5e145b0baaa72e51d104d21 schema:name doi
78 schema:value 10.1007/978-1-60327-993-2_4
79 rdf:type schema:PropertyValue
80 N9e631e69cd41452c8b4248c254aa0a25 schema:name dimensions_id
81 schema:value pub.1036737590
82 rdf:type schema:PropertyValue
83 Naae459868d674c28a2b8e7a0fa9fb638 rdf:first sg:person.0653577454.35
84 rdf:rest N0468f6d34e774a1d9cd3c1747faccb37
85 Nb72c4f1c94f043929337b7a465edf500 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Saccharomyces cerevisiae
87 rdf:type schema:DefinedTerm
88 Nb87fce91790f4cceb67fa8039792e2f4 schema:name readcube_id
89 schema:value af00eaf1505f7ec499b44d45c35c71b39fb432d57e575e1547286542c5e6a3d3
90 rdf:type schema:PropertyValue
91 Nc97f3daf4a6f489f89d46d40bb7f3626 rdf:first Nd35a317e97ad41b0abfbdd8be19fc72f
92 rdf:rest rdf:nil
93 Nd35a317e97ad41b0abfbdd8be19fc72f schema:familyName McAinsh
94 schema:givenName Andrew D.
95 rdf:type schema:Person
96 Nd99752e3d635442e91525e1ee074584e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Plasmids
98 rdf:type schema:DefinedTerm
99 Nfd0767e2e8684470a9e30fd0dcefbafe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Saccharomyces cerevisiae Proteins
101 rdf:type schema:DefinedTerm
102 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
103 schema:name Biological Sciences
104 rdf:type schema:DefinedTerm
105 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
106 schema:name Biochemistry and Cell Biology
107 rdf:type schema:DefinedTerm
108 sg:person.010107334027.29 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
109 schema:familyName Spanos
110 schema:givenName Adonis
111 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010107334027.29
112 rdf:type schema:Person
113 sg:person.015016201267.68 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
114 schema:familyName Sedgwick
115 schema:givenName Steven
116 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015016201267.68
117 rdf:type schema:Person
118 sg:person.0653577454.35 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
119 schema:familyName Geymonat
120 schema:givenName Marco
121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653577454.35
122 rdf:type schema:Person
123 sg:pub.10.1038/19775 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003128483
124 https://doi.org/10.1038/19775
125 rdf:type schema:CreativeWork
126 https://app.dimensions.ai/details/publication/pub.1082809367 schema:CreativeWork
127 https://doi.org/10.1016/j.cell.2005.04.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052815216
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1016/j.gene.2007.05.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030354208
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1016/s0092-8674(00)80733-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043251016
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1074/jbc.m011689200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025589174
134 rdf:type schema:CreativeWork
135 https://www.grid.ac/institutes/grid.451388.3 schema:alternateName The Francis Crick Institute
136 schema:name Stem Cell Biology and Developmental Genetics, National Institute for Medical Research, London, UK
137 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...