Duplex Unwinding and RNP Remodeling With RNA Helicases View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2008

AUTHORS

Eckhard Jankowsky , Margaret E. Fairman

ABSTRACT

RNA helicases are essential for the adenosine 5′-triphosphate (ATP)-driven rearrangement of many RNAs and RNA-protein complexes (ribonucleoproteins, RNPs) throughout RNA metabolism. We describe assays to measure RNA and RNP remodeling by RNA helicases in vitro. We show how to prepare substrates for these reactions and how to monitor unwinding of RNA duplexes and displacement of proteins from RNA using standard molecular biology techniques. More... »

PAGES

343-355

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60327-475-3_22

DOI

http://dx.doi.org/10.1007/978-1-60327-475-3_22

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029841022

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18982301


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