Synthesis of Peptide Arrays Using SPOT-Technology and the CelluSpots-Method View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009

AUTHORS

Dirk F.H. Winkler , Kai Hilpert , Ole Brandt , Robert E.W. Hancock

ABSTRACT

Peptide synthesis on cellulose using the SPOT technology follows the standard Fmoc-chemistry and can be performed manually or automated. This method allows the synthesis of low-cost peptide arrays containing around 900 large spots of addressable peptides on a cellulose sheet of 19 cm x 29 cm. These peptides can be cleaved from the cellulose support by ammonia gas and afterward spotted on glass microchips. Alternatively, the peptides can be synthesized on modified cellulose discs and CelluSpot microarrays can be produced. More... »

PAGES

157-174

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60327-394-7_5

DOI

http://dx.doi.org/10.1007/978-1-60327-394-7_5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028219316

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19649591


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