Fluorescent Biosensors to Investigate Helicase Activity View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009-09-15

AUTHORS

Martin R. Webb

ABSTRACT

ATP-driven translocation of helicases along DNA can be assayed in several ways. Reagentless biosensors, based on fluorophore-protein adducts, provide convenient ways for real-time assays of both the separation of dsDNA and the hydrolysis of ATP. Single-stranded DNA can be assayed using a modified single-stranded DNA-binding protein (SSB), and phosphate production during ATP hydrolysis can be measured by a modified phosphate-binding protein. Advantages and limitations of these approaches are compared with those of other types of measurements. More... »

PAGES

13-27

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60327-355-8_2

DOI

http://dx.doi.org/10.1007/978-1-60327-355-8_2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047802187

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20225139


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