Duplex Unwinding with DEAD-Box Proteins View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009-09-15

AUTHORS

Eckhard Jankowsky , Andrea Putnam

ABSTRACT

DEAD-box proteins, which comprise the largest helicase family, are involved in virtually all aspects of RNA metabolism. DEAD-box proteins catalyze diverse ATP-driven functions including the unwinding of RNA secondary structures. In contrast to many well-studied DNA and viral RNA helicases, DEAD-box proteins do not rely on translocation on one of the nucleic acid strands for duplex unwinding, but directly load onto helical regions and then locally pry the strands apart in an ATP-dependent fashion. In this chapter, we outline substrate design and unwinding protocols for DEAD-box proteins and focus on the quantitative evaluation of their unwinding activity. More... »

PAGES

245-264

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60327-355-8_18

DOI

http://dx.doi.org/10.1007/978-1-60327-355-8_18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014543546

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20225155


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