Construction of Phage Antibody Repertoires from the Blood of West Nile Virus-Infected Donors View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009-05-25

AUTHORS

Mark Throsby , John de Kruif

ABSTRACT

A method for the construction of West Nile virus immune donor antibody repertoires is described. B cells are harvested from a suitable donor and the antibody variable genes are amplified using polymerase chain reaction (PCR). The PCR fragments are cloned in a phage display vector to construct a repertoire that can be used in panning procedures to identify many unique monoclonal antibodies. More... »

PAGES

45-60

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60327-302-2_4

DOI

http://dx.doi.org/10.1007/978-1-60327-302-2_4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001901452

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19554286


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