Estimation of Disulfide Bonds Using Ellman’s Reagent View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1996

AUTHORS

Alastair Aitken , Michèle Learmonth

ABSTRACT

Ellman’s reagent 5,5′-dithiobis(2-nitrobenzoic acid)(DTNB) was first introduced in 1959 for the estimation of free thiol groups (1). The procedure is based on the reaction of the thiol with DTNB to give the mixed disulfide and 2-nitro-5-thiobenzoic acid (TNB) which is quantified by the absorbance of the anion (TNB2−) at 412 nm. More... »

PAGES

487-488

Book

TITLE

The Protein Protocols Handbook

ISBN

978-0-89603-338-2
978-1-60327-259-9

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60327-259-9_82

DOI

http://dx.doi.org/10.1007/978-1-60327-259-9_82

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052366668


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