Detection of Disulfide-Linked Peptides by HPLC View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1996

AUTHORS

Alastair Aitken , Michèle Learmonth

ABSTRACT

Classical techniques for determining disulfide bond patterns usually require the fragmentation of proteins into peptides under low-pH conditions to prevent disulphide exchange. Pepsin or cyanogen bromide are particularly useful (see Chapter 63). Diagonal techniques to identify disulphide-linked peptides were developed by Brown and Hartley (see Chapter 81). A modern micromethod employing reverse-phase HPLC is described here. More... »

PAGES

479-480

Book

TITLE

The Protein Protocols Handbook

ISBN

978-0-89603-338-2
978-1-60327-259-9

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60327-259-9_80

DOI

http://dx.doi.org/10.1007/978-1-60327-259-9_80

DIMENSIONS

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