Carboxymethylation of Cysteine Using Iodoacetamide/Iodoacetic Acid View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1996

AUTHORS

Alastair Aitken , Michèle Learmonth

ABSTRACT

If cysteine or cystine are identified in a protein, they require modification if they are to be quantified. Thiol groups may be blocked by a variety of reagents, including iodoacetic acid and iodoacetamide. Iodoacetate produces the S-carboxymethyl derivative of cysteine, effectively introducing new negative charges into the protein. Where such a charge difference is undesirable, iodoacetamide may be used to produce S-carboxyamidomethylcysteine (on acid hydrolysis, as for amino acid analysis, this yields S-carboxymethylcysteine). The charge difference between these two derivatives has been utilized in a method to quantify the number of cysteine residues in a protein ([1], see Chapter 83). More... »

PAGES

339-340

References to SciGraph publications

Book

TITLE

The Protein Protocols Handbook

ISBN

978-0-89603-338-2
978-1-60327-259-9

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-60327-259-9_51

DOI

http://dx.doi.org/10.1007/978-1-60327-259-9_51

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050787246


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "National Institute for Medical Research, Mill Hill, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Aitken", 
        "givenName": "Alastair", 
        "id": "sg:person.01152554741.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01152554741.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "National Institute for Medical Research, Mill Hill, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Learmonth", 
        "givenName": "Mich\u00e8le", 
        "id": "sg:person.0763337065.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0763337065.94"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/284487a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051310719", 
          "https://doi.org/10.1038/284487a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996", 
    "datePublishedReg": "1996-01-01", 
    "description": "If cysteine or cystine are identified in a protein, they require modification if they are to be quantified. Thiol groups may be blocked by a variety of reagents, including iodoacetic acid and iodoacetamide. Iodoacetate produces the S-carboxymethyl derivative of cysteine, effectively introducing new negative charges into the protein. Where such a charge difference is undesirable, iodoacetamide may be used to produce S-carboxyamidomethylcysteine (on acid hydrolysis, as for amino acid analysis, this yields S-carboxymethylcysteine). The charge difference between these two derivatives has been utilized in a method to quantify the number of cysteine residues in a protein ([1], see Chapter 83).", 
    "editor": [
      {
        "familyName": "Walker", 
        "givenName": "John M.", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-60327-259-9_51", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-0-89603-338-2", 
        "978-1-60327-259-9"
      ], 
      "name": "The Protein Protocols Handbook", 
      "type": "Book"
    }, 
    "name": "Carboxymethylation of Cysteine Using Iodoacetamide/Iodoacetic Acid", 
    "pagination": "339-340", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-60327-259-9_51"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "f1edf029811d4e42ed439fb3e36f3ecb41f4acee7e37efa92187e6693fd8555f"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1050787246"
        ]
      }
    ], 
    "publisher": {
      "location": "Totowa, NJ", 
      "name": "Humana Press", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-60327-259-9_51", 
      "https://app.dimensions.ai/details/publication/pub.1050787246"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-16T06:10", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000350_0000000350/records_77564_00000000.jsonl", 
    "type": "Chapter", 
    "url": "https://link.springer.com/10.1007%2F978-1-60327-259-9_51"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-259-9_51'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-259-9_51'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-259-9_51'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-60327-259-9_51'


 

This table displays all metadata directly associated to this object as RDF triples.

76 TRIPLES      23 PREDICATES      28 URIs      20 LITERALS      8 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-60327-259-9_51 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Nb8c90e876369453ca276d88c524b8939
4 schema:citation sg:pub.10.1038/284487a0
5 schema:datePublished 1996
6 schema:datePublishedReg 1996-01-01
7 schema:description If cysteine or cystine are identified in a protein, they require modification if they are to be quantified. Thiol groups may be blocked by a variety of reagents, including iodoacetic acid and iodoacetamide. Iodoacetate produces the S-carboxymethyl derivative of cysteine, effectively introducing new negative charges into the protein. Where such a charge difference is undesirable, iodoacetamide may be used to produce S-carboxyamidomethylcysteine (on acid hydrolysis, as for amino acid analysis, this yields S-carboxymethylcysteine). The charge difference between these two derivatives has been utilized in a method to quantify the number of cysteine residues in a protein ([1], see Chapter 83).
8 schema:editor Nb769290150d24d1ea8f4b0bcc1655c02
9 schema:genre chapter
10 schema:inLanguage en
11 schema:isAccessibleForFree false
12 schema:isPartOf N2e4fec46449c452c9475e7c5ddf4320b
13 schema:name Carboxymethylation of Cysteine Using Iodoacetamide/Iodoacetic Acid
14 schema:pagination 339-340
15 schema:productId N111cc055eda44523b3b71bd398f25b62
16 N4a72ce3be2c64923b0d11f4c1a87b69a
17 Nf1f14f06e33a4812bdbdc1b42e632b23
18 schema:publisher N2931150b347b48af966fe555d6634334
19 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050787246
20 https://doi.org/10.1007/978-1-60327-259-9_51
21 schema:sdDatePublished 2019-04-16T06:10
22 schema:sdLicense https://scigraph.springernature.com/explorer/license/
23 schema:sdPublisher Nf0323458289349fa87f3e5aaccacda8e
24 schema:url https://link.springer.com/10.1007%2F978-1-60327-259-9_51
25 sgo:license sg:explorer/license/
26 sgo:sdDataset chapters
27 rdf:type schema:Chapter
28 N111cc055eda44523b3b71bd398f25b62 schema:name doi
29 schema:value 10.1007/978-1-60327-259-9_51
30 rdf:type schema:PropertyValue
31 N2931150b347b48af966fe555d6634334 schema:location Totowa, NJ
32 schema:name Humana Press
33 rdf:type schema:Organisation
34 N2e4fec46449c452c9475e7c5ddf4320b schema:isbn 978-0-89603-338-2
35 978-1-60327-259-9
36 schema:name The Protein Protocols Handbook
37 rdf:type schema:Book
38 N3186e5fae27244cf98384a3476cde084 schema:familyName Walker
39 schema:givenName John M.
40 rdf:type schema:Person
41 N4a72ce3be2c64923b0d11f4c1a87b69a schema:name dimensions_id
42 schema:value pub.1050787246
43 rdf:type schema:PropertyValue
44 N4e3ca014e1414b9eafc6387e80bb7f0f rdf:first sg:person.0763337065.94
45 rdf:rest rdf:nil
46 Nb769290150d24d1ea8f4b0bcc1655c02 rdf:first N3186e5fae27244cf98384a3476cde084
47 rdf:rest rdf:nil
48 Nb8c90e876369453ca276d88c524b8939 rdf:first sg:person.01152554741.55
49 rdf:rest N4e3ca014e1414b9eafc6387e80bb7f0f
50 Nf0323458289349fa87f3e5aaccacda8e schema:name Springer Nature - SN SciGraph project
51 rdf:type schema:Organization
52 Nf1f14f06e33a4812bdbdc1b42e632b23 schema:name readcube_id
53 schema:value f1edf029811d4e42ed439fb3e36f3ecb41f4acee7e37efa92187e6693fd8555f
54 rdf:type schema:PropertyValue
55 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
56 schema:name Biological Sciences
57 rdf:type schema:DefinedTerm
58 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
59 schema:name Biochemistry and Cell Biology
60 rdf:type schema:DefinedTerm
61 sg:person.01152554741.55 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
62 schema:familyName Aitken
63 schema:givenName Alastair
64 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01152554741.55
65 rdf:type schema:Person
66 sg:person.0763337065.94 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
67 schema:familyName Learmonth
68 schema:givenName Michèle
69 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0763337065.94
70 rdf:type schema:Person
71 sg:pub.10.1038/284487a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051310719
72 https://doi.org/10.1038/284487a0
73 rdf:type schema:CreativeWork
74 https://www.grid.ac/institutes/grid.451388.3 schema:alternateName The Francis Crick Institute
75 schema:name National Institute for Medical Research, Mill Hill, London, UK
76 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...