Organotypic Entorhino-Hippocampal Slice Cultures—A Tool to Study the Molecular and Cellular Regulation of Axonal Regeneration and Collateral Sprouting In Vitro View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2007

AUTHORS

Domenico Del Turco , Thomas Deller

ABSTRACT

Organotypic slice cultures of the brain are widely used as a tool to study fundamental questions in neuroscience. In this chapter, we focus on a protocol based on organotypic slice cultures of mouse entorhinal cortex and hippocampus that can be employed to study axonal regeneration and collateral sprouting in the central nervous system in vitro. Using pharmacological as well as genetic approaches, axonal regeneration and sprouting can be influenced, and some of the molecular and cellular mechanisms involved in these processes can be identified. The protocol describes in detail (1) the generation of organotypic entorhino-hippocampal slice cultures, (2) the conditions needed for the analysis of axonal regeneration and collateral sprouting, respectively, (3) the lesioning technique, (4) tracing techniques to visualize regenerating entorhinal axons, and (5) an immunohistochemical technique to visualize sprouting fibers. More... »

PAGES

55-66

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-59745-504-6_5

DOI

http://dx.doi.org/10.1007/978-1-59745-504-6_5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029973655

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18309925


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