Fluorescent Transgenes to Study Interphase Chromosomes in Living Plants View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2008

AUTHORS

Antonius J. M. Matzke , Bruno Huettel , Johannes van der Winden , Marjori Matzke

ABSTRACT

Fluorescence tagging of genomic sites through the use of bacterial operator/repressor systems combined with fluorescent proteins permits high-resolution analysis of interphase chromosomes in living cells. This technique has been used to study interphase chromosome arrangement and dynamics in yeast, Drosophila, and mammalian cells, but is only beginning to be exploited in plant systems. In this chapter, we describe methods for producing and identifying Arabidopsis thaliana plants harbouring fluorescence-tagged transgenes. The use of these plants to analyze various aspects of interphase chromosome organization and dynamics in living cells using 3D wide-field fluorescence microscopy is discussed. Potential problems encountered when utilizing this technology in plants are considered. More... »

PAGES

241-265

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-59745-406-3_16

DOI

http://dx.doi.org/10.1007/978-1-59745-406-3_16

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045831480

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18951172


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