Generation, Culture and Flow-Cytometric Characterization of Primary Mouse Macrophages View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009

AUTHORS

Ulrike Schleicher , Christian Bogdan

ABSTRACT

Macrophages are not only host cells for many pathogens, but also fulfill several key functions in the innate and adaptive immune response, including the release of pro- and anti-inflammatory cytokines, the generation of organic and inorganic autacoids, the phagocytosis and killing of intracellular microorganisms or tumor cells, and the degradation and presentation of antigens. Several of these functions are shared by other immune cells, including dendritic cells, granulocytes, NK cells, and/or T lymphocytes. Thus, the analysis of macrophage functions in vitro using primary mouse cell populations requires standardized methods for the generation and culture of macrophages that guarantee high cell purity as well as the absence of stimulatory microbial contaminants. This chapter presents methodology to achieve these aims. More... »

PAGES

203-24

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-59745-396-7_14

DOI

http://dx.doi.org/10.1007/978-1-59745-396-7_14

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023323871

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19347320


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