Gene Insertion Patterns and Sites View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2008-10-07

AUTHORS

Philippe Vain , Vera Thole

ABSTRACT

During the past 25 years, the molecular analysis of transgene insertion patterns and sites in plants has greatly contributed to our understanding of the mechanisms underlying transgene integration, expression, and stability in the nuclear genome. Molecular characterization is also an essential step in the safety assessment of genetically modified crops. This chapter describes the standard experimental procedures used to analyze transgene insertion patterns and loci in cereals and grasses transformed using Agrobacterium tumefaciens or direct transfer of DNA. Methods and protocols enabling the determination of the number and configuration of transgenic loci via a combination of inheritance studies, polymerase chain reaction, and Southern analyses are presented. The complete characterization of transgenic inserts in plants is, however, a holistic process relying on a wide variety of experimental approaches. In this chapter, these additional approaches are not detailed but references to relevant bibliographic records are provided. More... »

PAGES

203-226

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-59745-379-0_13

DOI

http://dx.doi.org/10.1007/978-1-59745-379-0_13

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048118830

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19009448


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