Assessing Expression of Apoptotic Markers Using Large Cohort Tissue Microarrays View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2008

AUTHORS

Elah Pick , Mary M. McCarthy , Harriet M. Kluger

ABSTRACT

Apoptotic markers include proteins from the intrinsic and extrinsic pathways. These cascades include both pro-apoptotic and anti-apoptotic elements. The expression levels of these elements can be assessed by immunohistochemistry (IHC) and can indicate general trends in pro- versus anti-apoptotic tendencies of the cells. IHC is particularly useful when studying large cohorts of paraffin-embedded specimens. Advances in tissue microarray (TMA) technology have facilitated evaluation of large cohorts of specimens, as cores from hundreds of patients can be represented on a single glass slide and stained in a uniform fashion. In this chapter, we discuss construction and staining methods of TMAs and present examples of assessment of apoptotic marker expression in malignant and benign cells using a novel method of automated, quantitative analysis of in situ protein expression. More... »

PAGES

83-93

Book

TITLE

Apoptosis and Cancer

ISBN

978-1-58829-457-9
978-1-59745-339-4

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-59745-339-4_8

DOI

http://dx.doi.org/10.1007/978-1-59745-339-4_8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028883513


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