Isolation, Expansion, and Differentiation of Mouse Skin-Derived Precursors View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009

AUTHORS

Karl J.L. Fernandes , Freda D. Miller

ABSTRACT

The isolation and experimental manipulation of multipotent precursors is of increasing therapeutic relevance. We recently reported the generation of cultures of Skin-derived Precursors (‘SKPs’), multipotent cells that can be isolated from the dermis of embryonic, neonatal, and adult rodent skin (1), and from adult human skin (2) SKPs have similarities to stem cells of the embryonic neural crest (3), and differentiate into a variety of neural and mesodermal cell phenotypes, including peripheral neurons and glial cells, smooth muscle cells, bone, cartilage, and adipocytes (3–5). Here, we detail the establishment, propagation, neural differentiation, and immunocytochemical analysis of SKP cultures. More... »

PAGES

159-170

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-59745-060-7_10

DOI

http://dx.doi.org/10.1007/978-1-59745-060-7_10

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026699312

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19089355


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