Use of Gas-Phase Fractionation to Increase Protein Identifications View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2008

AUTHORS

Jacob Kennedy , Eugene C. Yi

ABSTRACT

Gas-phase fractionation (GPF), defined as iterative mass spectrometric interrogations of a sample over multiple smaller mass-to-charge (m/z) ranges, enables the ions selected for collision-induced dissociation to come from a greater number of unique peptides compared to the ions selected from the wide mass range scan in automated liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. GPF is described as a means to achieve higher proteome coverage than multiple LC-MS/MS analyses of unfractionated complex peptide mixtures. It is applied to organellar proteomics through analysis of yeast peroxisomal proteins obtained from a discontinuous Nycodenz gradient fraction known to be enriched with yeast peroxisomal membrane proteins. More... »

PAGES

217-228

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-59745-028-7_15

DOI

http://dx.doi.org/10.1007/978-1-59745-028-7_15

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007300574

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18370021


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