Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2010-02-08

AUTHORS

Henrik Ahlenius , Zaal Kokaia

ABSTRACT

Neural stem cells are defined as cells that either gives rise to or derives from the cells of the central nervous system and have the unique properties of stem cells, i.e. self-renewal and multipotentiality. One of the widely used methods of expanding neural stem cells under culture conditions is based on the capacity of these cells to divide continuously when cultured in serum-free medium supplemented with various growth factors. One common method used is to grow neural stem cells as free-floating aggregates of cells called neurospheres. Neurospheres can be generated from several structures of the embryonic and adult mammalian brain. Although viable lines can be generated from crude extracts of brain, a precise dissection is crucial to get a pure population of cells. Here we describe methods for dissection, isolation and generation of neurospheres from embryonic ganglionic eminences and adult subventricular zone of mice and rats. More... »

PAGES

241-252

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-59745-019-5_18

DOI

http://dx.doi.org/10.1007/978-1-59745-019-5_18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012178746

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20204633


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